Temporal and spatial resolution of distal protein motions that activate hydrogen tunneling in soybean lipoxygenase

JPT Zaragoza, AR Offenbacher, S Hu… - Proceedings of the …, 2023 - National Acad Sciences
The enzyme soybean lipoxygenase (SLO) provides a prototype for deep tunneling
mechanisms in hydrogen transfer catalysis. This work combines room temperature X-ray …

Building better polymerases: Engineering the replication of expanded genetic alphabets

Z Ouaray, SA Benner, MM Georgiadis… - Journal of Biological …, 2020 - ASBMB
DNA polymerases are today used throughout scientific research, biotechnology, and
medicine, in part for their ability to interact with unnatural forms of DNA created by synthetic …

Fatty acid allosteric regulation of CH activation in plant and animal lipoxygenases

AR Offenbacher, TR Holman - Molecules, 2020 - mdpi.com
Lipoxygenases (LOXs) catalyze the (per) oxidation of fatty acids that serve as important
mediators for cell signaling and inflammation. These reactions are initiated by a CH …

Effect of solvent viscosity on the activation barrier of hydrogen tunneling in the lipoxygenase reaction

L Guevara, M Gouge, A Ohler, SG Hill, S Patel… - Archives of Biochemistry …, 2023 - Elsevier
Hydrogen tunneling in enzyme reactions has played an important role in linking protein
thermal motions to the chemical steps of catalysis. Lipoxygenases (LOXs) have served as …

Impact of N-Glycosylation on Protein Structure and Dynamics Linked to Enzymatic C–H Activation in the M. oryzae Lipoxygenase

C Whittington, A Sharma, SG Hill, AT Iavarone… - Biochemistry, 2024 - ACS Publications
Lipoxygenases (LOXs) from pathogenic fungi are potential therapeutic targets for defense
against plant and select human diseases. In contrast to the canonical LOXs in plants and …

Arachidonic acid: reconciling the dichotomy of its oxidative cascade through specific deuteration

JT Brenna, MG Sergeeva, NB Pestov… - Free Radical …, 2024 - Taylor & Francis
A new approach to attenuating pathological inflammatory reactions by buffering the
eicosanoid pathways with oxidation-resistant hexadeuterated arachidonic acid (D-ARA) is …

Temporal and spatial resolution of a protein quake that activates hydrogen tunneling in soybean lipoxygenase

JPT Zaragoza, AR Offenbacher, S Hu, CL Gee… - bioRxiv, 2022 - biorxiv.org
The enzyme soybean lipoxygenase (SLO) provides a prototype for deep tunneling
mechanisms in hydrogen transfer catalysis. This work combines room temperature X-ray …

Photothermal Methanation of CO with Benzyl Alcohol as a Hydrogen Source Using Au–Ni Alloy Catalyst

Y Zhou, Y Yang, Y Hu - Korean Journal of Chemical Engineering, 2024 - Springer
The CO methanation process finds extensive application in natural gas synthesis, typically
relying on hydrogen as the primary reducing agent. However, due to significant …

Investigating the rate-limiting step of mitochondrial complex I catalysis

MN Choy - 2024 - repository.cam.ac.uk
Respiratory complex I (NADH: ubiquinone oxidoreductase) is a key enzyme in metabolism
and is the least understood protein in the mitochondrial electron transfer chain (ETC). It …

Effective Photothermal Co Hydrogenation Enabled By Au-Ni Alloy Catalyst

Y Zhou, H Deng, X Wang, Y Hu - … Co Hydrogenation Enabled By Au-Ni … - papers.ssrn.com
The CO methanation process is commonly utilized in natural gas synthesis, where hydrogen
is often used as the main source. However, this study aimed to improve CO hydrogenation …