The diversification of methods for studying cell–cell interactions and communication

E Armingol, HM Baghdassarian, NE Lewis - Nature Reviews Genetics, 2024 - nature.com
No cell lives in a vacuum, and the molecular interactions between cells define most
phenotypes. Transcriptomics provides rich information to infer cell–cell interactions and …

Hallmarks of stemness in mammalian tissues

J Beumer, H Clevers - Cell Stem Cell, 2024 - cell.com
All adult tissues experience wear and tear. Most tissues can compensate for cell loss
through the activity of resident stem cells. Although the cellular maintenance strategies vary …

Decoding the Complexity of Immune–Cancer Cell Interactions: Empowering the Future of Cancer Immunotherapy

K Maffuid, Y Cao - Cancers, 2023 - mdpi.com
Simple Summary Cell-to-cell communication between the immune system and tumors is of
the utmost importance; it influences the development of tumors, their growth, and how they …

Single-cell sequencing to multi-omics: Technologies and applications

X Wu, X Yang, Y Dai, Z Zhao, J Zhu, H Guo, R Yang - Biomarker Research, 2024 - Springer
Cells, as the fundamental units of life, contain multidimensional spatiotemporal information.
Single-cell RNA sequencing (scRNA-seq) is revolutionizing biomedical science by …

Creative approaches using proximity labeling to gain new biological insights

RR Milione, BB Schell, CJ Douglas… - Trends in Biochemical …, 2024 - cell.com
At its most fundamental level, life is a collection of synchronized cellular processes driven by
interactions among biomolecules. Proximity labeling has emerged as a powerful technique …

Adult tissue-specific stem cell interaction: novel technologies and research advances

X Luo, Z Liu, R Xu - Frontiers in Cell and Developmental Biology, 2023 - frontiersin.org
Adult tissue-specific stem cells play a dominant role in tissue homeostasis and regeneration.
Various in vivo markers of adult tissue-specific stem cells have been increasingly reported …

Ru(bpy)32+‐Enabled Cell‐Surface Photocatalytic Proximity Labeling toward More Efficient Capture of Physically Interacting Cells

S Qiu, W Li, T Deng, A Bi, Y Yang, X Jiang… - Angewandte …, 2023 - Wiley Online Library
Intercellular proximity labeling has emerged as a promising approach to enable the study of
cell‐cell interactions (CCIs), but the efficiency of current platforms is limited. Here, we use Ru …

Glycosyltransferases as versatile tools to study the biology of glycans

JM Kofsky, JL Babulic, ME Boddington… - …, 2023 - academic.oup.com
All cells are decorated with complex carbohydrate structures called glycans that serve as
ligands for glycan-binding proteins (GBPs) to mediate a wide range of biological processes …

Towards chemoenzymatic labeling strategies for profiling protein glycosylation

Y Tian, S Ma, L Wen - Current Opinion in Chemical Biology, 2024 - Elsevier
Protein glycosylation is one of the most common and important post-translational
modifications of proteins involved in regulating glycoprotein functions. The chemoenzymatic …

Droplet-based functional CRISPR screening of cell–cell interactions by SPEAC-seq

C Faust Akl, M Linnerbauer, Z Li, HG Lee, IC Clark… - Nature …, 2024 - nature.com
Cell–cell interactions are essential for the function and contextual regulation of biological
tissues. We present a platform for high-throughput microfluidics-supported genetic screening …