Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy

G de Vito, L Turrini, C Müllenbroich, P Ricci… - Biomedical optics …, 2022 - opg.optica.org
Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional
imaging in zebrafish larvae. Conventional one-photon LSFM can however induce …

Effects of excitation light polarization on fluorescence emission in two-photon light-sheet microscopy

G de Vito, P Ricci, L Turrini, V Gavryusev… - Biomedical Optics …, 2020 - opg.optica.org
Light-sheet microscopy (LSM) is a powerful imaging technique that uses a planar
illumination oriented orthogonally to the detection axis. Two-photon (2P) LSM is a variant of …

Multimodal Characterization of Seizures in Zebrafish Larvae

L Turrini, M Sorelli, G de Vito, C Credi, N Tiso, F Vanzi… - Biomedicines, 2022 - mdpi.com
Epilepsy accounts for a significant proportion of the world's disease burden. Indeed, many
research efforts are produced both to investigate the basic mechanism ruling its genesis and …

Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity

L Turrini, P Ricci, M Sorelli, G de Vito… - Communications …, 2024 - nature.com
One of the most audacious goals of modern neuroscience is unraveling the complex web of
causal relations underlying the activity of neuronal populations on a whole-brain scale. This …

Reconstruction scheme for excitatory and inhibitory dynamics with quenched disorder: application to zebrafish imaging

L Chicchi, G Cecchini, I Adam, G de Vito, R Livi… - Journal of …, 2021 - Springer
An inverse procedure is developed and tested to recover functional and structural
information from global signals of brains activity. The method assumes a leaky-integrate and …

Two-photon light-sheet microscopy for high-speed whole-brain functional imaging of zebrafish neuronal physiology and pathology

G de Vito, L Turrini, C Fornetto, P Ricci… - …, 2020 - spiedigitallibrary.org
We present the development of a custom-made two-photon light-sheet microscope
optimized for high-speed (5 Hz) volumetric imaging of zebrafish larval brain for the analysis …

Direct activation of zebrafish neurons by ultrasonic stimulation revealed by whole CNS calcium imaging

N Meneghetti, F Dedola, V Gavryusev… - Journal of Neural …, 2020 - iopscience.iop.org
Objective. Ultrasounds (US) use in neural engineering is so far mainly limited to ablation
through high intensity focused ultrasound, but interesting preliminary results show that low …

Two-photon all-optical electrophysiology for the dissection of larval zebrafish brain functional connectivity

L Turrini, P Ricci, M Sorelli, G de Vito, M Marchetti… - bioRxiv, 2024 - biorxiv.org
One of the most audacious goals of modern neuroscience is unraveling the complex web of
causal relations underlying the activity of neuronal populations on a whole-brain scale. This …

Brain-wide functional imaging to highlight differences between the diurnal and nocturnal neuronal activity in zebrafish larvae

G de Vito, L Turrini, C Fornetto, E Trabalzini… - arXiv preprint arXiv …, 2023 - arxiv.org
Most living organisms show highly conserved physiological changes following a 24-hour
cycle which goes by the name of circadian rhythm. Among experimental models, the effects …

Fast multi-photon brain-wide volumetric imaging and photostimulation

L Turrini, P Ricci, G de Vito, M Sorelli… - … Microscopy in the …, 2023 - spiedigitallibrary.org
The ability to simultaneously detect and control neuronal activity grants the capacity to
unravel the causal relationships behind brain processing. Nevertheless, scaling-up this …