Controlled protein crystal nucleation in microreactors: the effect of the droplet volume versus high supersaturation ratios

J Ferreira, F Castro, S Kuhn, F Rocha - CrystEngComm, 2020 - pubs.rsc.org
Herein, the control of enhanced protein nucleation in microdroplets under high
supersaturation ratios is discussed, where lysozyme is used as a model protein. This study …

Kinetic factors may reshape the dependence of crystal nucleation rate on temperature in protein bulk solution

IL Dimitrov - Journal of Biological Physics, 2020 - Springer
Here we provide an analysis of primary results obtained from a study of apoferritin crystal
nucleation in compositionally invariant bulk solution at constant supersaturation ratio of the …

Temperature-dependent growth of protein crystals with temperature-independent solubility: case study of apoferritin

IL Dimitrov - CrystEngComm, 2020 - pubs.rsc.org
The crystal growth of apoferritin, a protein with negligible temperature dependence of
solubility, was investigated in the temperature range of 10–60° C. An approach which …

Protein crystallization in droplet-based microsystems

J Ferreira - 2020 - search.proquest.com
The development of the first microfluidic prototype appeared at the beginning of the 1980s.
However, over the last decade, droplet-based microfluidics has emerged as a powerful tool …