This paper aims to discuss various aspects of the use of reference genes in qPCR technique
used in the thousands of present studies. Most frequently, these are housekeeping genes …

Abstract In 1941, Beadle and Tatum published experiments that would explain the basis of
the central dogma of molecular biology, whereby the DNA through an intermediate …

Real-time reverse transcription PCR (RT-qPCR) has emerged as an accurate and widely
used technique for expression profiling of selected genes. However, obtaining reliable …

Reverse transcription quantitative real-time PCR (RT-qPCR) is a sensitive technique for
quantifying gene expression, but its success depends on the stability of the reference gene …

Drosophila melanogaster is one of the most important genetic models and techniques such
as reverse transcription quantitative real-time PCR (RT-qPCR) are being employed …

Red flower color has arisen multiple times and is generally associated with hummingbird
pollination. The majority of evolutionary transitions to red color proceeded from purple …

Background Rice plant growth is comprised of distinct phases, such as vegetative,
reproductive, grain filling and maturity phases. In these phases synthesis and availability of …

Members of the R2R3‐MYB transcription factor subgroup 19 (SG19) have been extensively
studied in multiple plant species using different silenced or mutated lines. Some studies …

Background Real-time quantitative PCR (RT-qPCR) is the most commonly used method for
accurately detecting gene expression patterns. As part of RT-qPCR analysis, normalization …

Real-time reverse transcription PCR (RT-qPCR) is a preferred method for rapid and
accurate quantification of gene expression studies. Appropriate application of RT-qPCR …