The long-dreamed-of capability of monitoring the molecular machinery in living systems has
not been realized yet, mainly due to the technical limitations of current sensing technologies …

Paramagnetic chemical probes have been used in electron paramagnetic resonance (EPR)
and nuclear magnetic resonance (NMR) spectroscopy for more than four decades. Recent …

Membrane proteins possess a variety of functions essential to the survival of organisms.
However, due to their inherent hydrophobic nature, it is extremely difficult to probe the …

Site‐directed spin labeling (SDSL) in combination with electron paramagnetic resonance
(EPR) spectroscopy is a rapidly expanding powerful biophysical technique to study the …

Within the microbial rhodopsin family, heliorhodopsins (HeRs) form a phylogenetically
distinct group of light-harvesting retinal proteins with largely unknown functions. We have …

Pulse electron paramagnetic resonance measurements of nanometer scale distance
distributions have proven highly effective in structural studies. They exploit the magnetic …

Biological membranes are essential in providing the stability of membrane proteins in a
functional state. Functionally stable homogeneous sample is required for biophysical …

In-cell distance determination by electron paramagnetic resonance (EPR) spectroscopy
reveals essential structural information about biomacromolecules under native conditions …

Background Specific interests in improving the functionality of food proteins as well as in
protein‒food constituent interactions have resulted in a tremendous number of scientific …

In cases of subcutaneous injection of therapeutic monoclonal antibodies, high protein
concentrations (> 50 mg/ml) are often required. During the development of these high …