Next-generation sequencing for biodefense: biothreat detection, forensics, and the clinic

TD Minogue, JW Koehler, CP Stefan… - Clinical …, 2019 - academic.oup.com
BACKGROUND Next-generation sequencing (NGS) is revolutionizing a variety of molecular
biology fields including bioforensics, biosurveillance, and infectious disease diagnostics. For …

Highly multiplexed targeted sequencing strategy for infectious disease surveillance

I Hernández-Neuta, A Magoulopoulou, F Pineiro… - BMC …, 2023 - Springer
Background Global efforts to characterize diseases of poverty are hampered by lack of
affordable and comprehensive detection platforms, resulting in suboptimal allocation of …

Comparison of illumina and Oxford nanopore sequencing technologies for pathogen detection from clinical matrices using molecular inversion probes

CP Stefan, AT Hall, AS Graham, TD Minogue - The Journal of Molecular …, 2022 - Elsevier
Next-generation sequencing is rapidly finding footholds in numerous microbiological fields,
including infectious disease diagnostics. Here, we describe a molecular inversion probe …

DNA Fragment Enrichment for High-Throughput Sequencing

AN Sinyakov, EV Kostina - Molecular Biology, 2023 - Springer
This review describes the application of oligonucleotides, which are mainly obtained using
DNA synthesizers of a new generation (microarray DNA synthesizers), for the enrichment of …

Same-day simultaneous diagnosis of bacterial and fungal infections in clinical practice by nanopore targeted sequencing

M Wang, A Fu, B Hu, G Shen, R Liu, W Zhao, S Jiang… - MedRxiv, 2020 - medrxiv.org
BACKGROUND As approximately 19% of global deaths are attributable to infectious
diseases, early diagnosis of infection is very important to reduce mortality. Traditional …

Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction

T Maxson, CD Blancett, AS Graham, CP Stefan… - PLoS …, 2018 - journals.plos.org
Development and implementation of rapid antimicrobial susceptibility testing is critical for
guiding patient care and improving clinical outcomes, especially in cases of sepsis. One …

An improved molecular inversion probe based targeted sequencing approach for low variant allele frequency

T Biezuner, Y Brilon, AB Arye, B Oron… - NAR genomics and …, 2022 - academic.oup.com
Deep targeted sequencing technologies are still not widely used in clinical practice due to
the complexity of the methods and their cost. The Molecular Inversion Probes (MIP) …

Rapid detection of carbapenem-resistant Enterobacteriaceae using pH response based on vancomycin-modified Fe 3 O 4@ Au nanoparticle enrichment and the …

J Wang, W Yang, Q Peng, D Han, L Kong, L Fan… - Analytical …, 2020 - pubs.rsc.org
Rapid and simple detection of carbapenem-resistant Enterobacteriaceae (CRE) is important
to manage infection and prevent the spread of these bacteria. In this work, we utilize pH …

A Multi-Omic Approach to Evaluating Soybean Iron Deficiency Chlorosis in the Field

CJ Oswald - 2020 - search.proquest.com
Iron deficiency chlorosis (IDC) is an abiotic stress and yield limiting factor of soybean
[Glycine max (L.) Merrill] grown on calcareous, high pH soils throughout the North Central …

[PDF][PDF] Synergetic effects of Bacillus subtilis and antibiotics against Alcaligenes faecalis

NH Aldujaili, AS Hameed, SR Banoon - database - systems.uomisan.edu.iq
Aim: The use of beneficial bacteria in conjunction with antibiotics may decrease the effects of
dysbiosis initiated by the antibiotics, and maximize the assistance of probiotics in the gut in …