Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
Latest developed strategies to minimize the off-target effects in CRISPR-Cas-mediated genome editing
Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …
Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair
Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR associated
protein 9 (Cas9)-based therapeutics, especially those that can correct gene mutations via …
protein 9 (Cas9)-based therapeutics, especially those that can correct gene mutations via …
CRISPR–Cas9 structures and mechanisms
Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–
CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 …
CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 …
A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
Although polymerase chain reaction (PCR) is the most widely used method for DNA
amplification, the requirement of thermocycling limits its non-laboratory applications …
amplification, the requirement of thermocycling limits its non-laboratory applications …
CRISPR/Cas systems in genome editing: methodologies and tools for sgRNA design, off‐target evaluation, and strategies to mitigate off‐target effects
H Manghwar, B Li, X Ding, A Hussain… - Advanced …, 2020 - Wiley Online Library
Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory …
[HTML][HTML] R-loop formation and conformational activation mechanisms of Cas9
Cas9 is a CRISPR-associated endonuclease capable of RNA-guided, site-specific DNA
cleavage,–. The programmable activity of Cas9 has been widely utilized for genome editing …
cleavage,–. The programmable activity of Cas9 has been widely utilized for genome editing …
Kinetic basis for DNA target specificity of CRISPR-Cas12a
Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising
applications in human health and disease. However, DNA cleavage at off-target sites that …
applications in human health and disease. However, DNA cleavage at off-target sites that …
[HTML][HTML] Structural basis for Cas9 off-target activity
The target DNA specificity of the CRISPR-associated genome editor nuclease Cas9 is
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
determined by complementarity to a 20-nucleotide segment in its guide RNA. However …
Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor
S Balderston, JJ Taulbee, E Celaya, K Fung… - Nature Biomedical …, 2021 - nature.com
Simple and fast methods for the detection of target genes with single-nucleotide specificity
could open up genetic research and diagnostics beyond laboratory settings. We recently …
could open up genetic research and diagnostics beyond laboratory settings. We recently …