More than fifty years after the 3Rs definition and despite the continuous implementation of
regulatory measures, animals continue to be widely used in basic research. Their use …

In tissue engineering, scaffolding plays an important role in accommodating and stimulating
new tissue growth. Chitosan and alginate are two widely used natural polymers in tissue …

The bovine mammary gland has vital importance in the dairy sector, as it is considered a
source of basic dairy product, milk. Mammary gland affections are widespread, which affect …

Milk is a complex fluid required for development, nutrition and immunological protection to
the newborn offspring. Interestingly, latest finding proved the presence of novel stem cell …

Bone tissue engineering requires a combination of cells, efficient biochemical and
physicochemical factors, and biocompatible scaffolds. In this study, we evaluated the …

The goal of the dairy industry is ultimately to increase lactation persistency, which is the
length of time during which peak milk yield is sustained. Lactation persistency is determined …

Androgens are involved in maintaining epididymal structure and function. In the present
study, primary culture of goat EECs and effect of testosterone on expression of glutathione …

旨在建立绵羊附睾上皮永生化细胞系, 为进一步研究绵羊附睾功能调节机制提供基础.
本研究采用酶消化法分离培养原代绵羊附睾上皮细胞, 利用脂质体将pCI-neo-hTERT …

Simple Summary Milk fat is mainly composed of triglycerides, which are synthesized in the
endoplasmic reticulum of mammary epithelial cells. Many uncertain regulatory factors and …

本研究旨在建立一种简单易行, 获取细胞纯度高的绒山羊附睾上皮细胞体外培养体系. 10~ 12
月龄绒山羊附睾头部分别采用酶消化法和改良组织块消化法进行原代培养 …