Digital twins for tissue culture techniques—concepts, expectations, and state of the art

J Möller, R Pörtner - Processes, 2021 - mdpi.com
Techniques to provide in vitro tissue culture have undergone significant changes during the
last decades, and current applications involve interactions of cells and organoids, three …

Single-cell infrared microspectroscopy quantifies dynamic heterogeneity of mesenchymal stem cells during adipogenic differentiation

Y Wang, W Dai, Z Liu, J Liu, J Cheng, Y Li, X Li… - Analytical …, 2020 - ACS Publications
The central relevance of cellular heterogeneity to biological phenomena raises the rational
needs for analytical techniques with single-cell resolution. Here, we developed a single-cell …

Coupling of P2Y receptors to Ca2+ mobilization in mesenchymal stromal cells from the human adipose tissue

PD Kotova, MF Bystrova, OA Rogachevskaja… - Cell Calcium, 2018 - Elsevier
The purinergic transduction was examined in mesenchymal stromal cells (MSCs) from the
human adipose tissue, and several nucleotides, including ATP, UTP, and ADP, were found …

Heterogeneous structure of stem cells dynamics: statistical models and quantitative predictions

P Bogdan, BM Deasy, B Gharaibeh, T Roehrs… - Scientific reports, 2014 - nature.com
Understanding stem cell (SC) population dynamics is essential for developing models that
can be used in basic science and medicine, to aid in predicting cells fate. These models can …

Bistable epigenetic states explain age-dependent decline in mesenchymal stem cell heterogeneity

Z Hamidouche, K Rother, J Przybilla, A Krinner… - Stem Cells, 2017 - academic.oup.com
The molecular mechanisms by which heterogeneity, a major characteristic of stem cells, is
achieved are yet unclear. We here study the expression of the membrane stem cell antigen …

In vivo, in vitro, in silico: computational tools for product and process design in tissue engineering

L Geris - Computational Modeling in Tissue Engineering, 2012 - Springer
This chapter aims to provide an introduction to how engineering tools in general and
computational models in particular can contribute to advancing the tissue engineering (TE) …

[PDF][PDF] Digital twins for tissue culture techniques—concepts, expectations, and state of the art. Processes 2021; 9: 447

J Möller, R Pörtner - 2021 - psecommunity.org
Techniques to provide in vitro tissue culture have undergone significant changes during the
last decades, and current applications involve interactions of cells and organoids, three …

Calcium signaling initiated by agonists in mesenchymal stromal cells from the human adipose tissue

PD Kotova, OA Rogachevskaja… - Calcium and Signal …, 2018 - books.google.com
Mesenchymal stromal cells (MSCs) from different sources represent a heterogeneous
population of proliferating non-differentiated cells that contain multipotent stem cells capable …

cGMP-dependent protein kinase modulates the sensitivity of mesenchymal stromal cells to purinergic agonists

EN Kochkina, PD Kotova, NI Enukashvily… - … ), Supplement Series A …, 2019 - Springer
Calcium signaling induced by ATP in mesenchymal stromal cells (MSCs) from the human
adipose tissue was studied by using the Ca 2+ dye Fluo-4 and Ca 2+ imaging. Previously …

Computational Models of Spatio-temporal Stem Cell Organization

P Buske, J Galle - Stem Cell Biology and Regenerative Medicine, 2022 - taylorfrancis.com
A main objective of computational modeling approaches to stem cell organization is to
provide a general and predictive model framework that links observable phenomena on the …