Formation of mRNA 3′ ends in eukaryotes requires the interaction of transacting factors
with cis-acting signal elements on the RNA precursor by two distinct mechanisms, one for …

A poly (A) tail is found at the 3 end of nearly every fully processed eukaryotic mRNA and has
been suggested to influence virtually all aspects of mRNA metabolism. Its proposed …

Whereas T helper cells recognize peptide–major histocompatibility complex (MHC) class II
complexes through their T cell receptors (TCRs), CD4 binds to an antigen-independent …

Ends of almost all eukaryotic mRNAs are generated by endonucleolytic cleavage and
addition of a poly (A) tail. In mammalian cells, the reaction depends on the sequence …

In yeast, four factors (CF I, CF II, PF I, and PAP) are required for accurate pre-mRNA
cleavage and polyadenylation in vitro. CF I can be separated further into CF IA and CF IB …

RNA-specific nucleotidyl transferases (rNTrs) are a diverse family of template-independent
polymerases that add ribonucleotides to the 3′-ends of RNA molecules. All rNTrs share a …

During their synthesis in the cell nucleus, most eukaryotic mRNAs undergo a two‐step 3′‐
end processing reaction in which the pre‐mRNA is cleaved and released from the …

We have tested deletion and substitution mutants of bovine poly (A) polymerase, and have
identified a small region that overlaps with a nuclear localization signal and binds to the …

During the synthesis of mRNA in the nucleus, 3′-ends are generated by endonucleolytic
cleavage followed by polyadenylation. The machinery responsible for this simple reaction is …

Mammalian cells contain a highly specific terminal uridylyl transferase (TUTase) that
exclusively accepts U6 snRNA as substrate. This enzyme, termed U6-TUTase, was purified …