Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is
an interdisciplinary field that takes advantage of advances in biochemistry, physics, and …

Highlights•Cryogenic electron microscopy has been evolving towards a big data field.•Movie-
mode collection and automation has increased throughput.•Improved processing pipelines …

Since the beginning of electron microscopy, resolution has been a critical parameter. In this
article, we propose a fully automatic, accurate method for determining the local resolution of …

CRISPR adaptation immunizes bacteria and archaea against viruses. During adaptation, the
Cas1-Cas2 complex integrates fragments of invader DNA as spacers in the CRISPR array …

Cryo-electron microscopy (cryo-EM) maps usually show heterogeneous distributions of B-
factors and electron density occupancies and are typically B-factor sharpened to improve …

Cryo-electron microscopy (cryoEM) has become a well established technique to elucidate
the 3D structures of biological macromolecules. Projection images from thousands of …

Some molecular chaperones are involved not only in assisting the folding of proteins but
also, given appropriate conditions, in their degradation. This is the case for Hsp70 and …

Microscopy imaging, including fluorescence micro scopy and electron microscopy, has
taken a prominent role in life science research and medicine due to its ability to investigate …

Single-particle cryo-electron microscopy (cryo-EM) has recently become a mainstream
technique for the structural determination of macromolecules. Typical cryo-EM workflows …

Xmipp is an open-source software package consisting of multiple programs for processing
data originating from electron microscopy and electron tomography, designed and managed …