[HTML][HTML] High‐efficiency and multilocus targeted integration in CHO cells using CRISPR‐mediated donor nicking and DNA repair inhibitors
NK Hamaker, KH Lee - Biotechnology and Bioengineering, 2023 - Wiley Online Library
Efforts to leverage clustered regularly interspaced short palindromic repeats/CRISPR‐
associated protein 9 (CRISPR/Cas9) for targeted genomic modifications in mammalian cells …
associated protein 9 (CRISPR/Cas9) for targeted genomic modifications in mammalian cells …
Genomic barcoding for clonal diversity monitoring and control in cell-based complex antibody production
N Bauer, C Oberist, M Poth, J Stingele, O Popp… - Scientific Reports, 2024 - nature.com
Engineered mammalian cells are key for biotechnology by enabling broad applications
ranging from in vitro model systems to therapeutic biofactories. Engineered cell lines exist as …
ranging from in vitro model systems to therapeutic biofactories. Engineered cell lines exist as …
[HTML][HTML] Context-dependent genomic locus effects on antibody production in recombinant Chinese hamster ovary cells generated through random integration
High-yield production of therapeutic protein using Chinese hamster ovary (CHO) cells
requires stable cell line development (CLD). CLD typically uses random integration of …
requires stable cell line development (CLD). CLD typically uses random integration of …
A systemic approach to identifying sequence frameworks that decrease mAb production in a transient Chinese hamster ovary cell expression system
AC Szkodny, KH Lee - Biotechnology Progress, 2024 - Wiley Online Library
Monoclonal antibodies (mAbs) are often engineered at the sequence level for improved
clinical performance yet are rarely evaluated prior to candidate selection for their …
clinical performance yet are rarely evaluated prior to candidate selection for their …
Revisiting the impact of genomic hot spots: C12orf35 locus as a hot spot and engineering target
K Cho, JS Lee - Biotechnology and Bioengineering, 2024 - Wiley Online Library
Traditional Chinese hamster ovary (CHO) cell line development is based on random
integration (RI) of transgene that causes clonal variation and subsequent large‐scale clone …
integration (RI) of transgene that causes clonal variation and subsequent large‐scale clone …
Evaluation of two transposases for improving expression of recombinant proteins in Chinese hamster ovary cell stable pools by co‐transfection and supertransfection …
Transposons are genetic elements capable of cutting and pasting genes of interest via the
action of a transposase and offer many advantages over random or targeted integration of …
action of a transposase and offer many advantages over random or targeted integration of …
[图书][B] Investigating “Difficult-to-Express” Monoclonal Antibody Frameworks in Transient and Site-Specific Integration-Based Chinese Hamster Ovary Cell Expression …
AC Szkodny - 2023 - udspace.udel.edu
Monoclonal antibodies (mAbs), the dominant modality in the growing biopharmaceutical
(biopharma) market, can exhibit undesired “developability” characteristics that affect their …
(biopharma) market, can exhibit undesired “developability” characteristics that affect their …
[引用][C] Advanced cell technologies: Making protein, cell, and gene therapies a reality
I Geada, A Roldão, MJ Betenbaugh… - Biotechnology and …, 2023 - Wiley Online Library
Researchers and engineers came together in Lisbon at the 27th Meeting of the European
Society for Animal Cell Technology (ESACT 2022), to discuss the latest advances in …
Society for Animal Cell Technology (ESACT 2022), to discuss the latest advances in …