Cell-free gene expression: an expanded repertoire of applications

AD Silverman, AS Karim, MC Jewett - Nature Reviews Genetics, 2020 - nature.com
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

[HTML][HTML] Cell-free gene expression

D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

Emerging droplet microfluidics

L Shang, Y Cheng, Y Zhao - Chemical reviews, 2017 - ACS Publications
Droplet microfluidics generates and manipulates discrete droplets through immiscible
multiphase flows inside microchannels. Due to its remarkable advantages, droplet …

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Droplet based microfluidics

R Seemann, M Brinkmann, T Pfohl… - Reports on progress …, 2011 - iopscience.iop.org
Droplet based microfluidics is a rapidly growing interdisciplinary field of research combining
soft matter physics, biochemistry and microsystems engineering. Its applications range from …

MaxSynBio: avenues towards creating cells from the bottom up

P Schwille, J Spatz, K Landfester… - Angewandte Chemie …, 2018 - Wiley Online Library
A large German research consortium mainly within the Max Planck Society (“MaxSynBio”)
was formed to investigate living systems from a fundamental perspective. The research …

Droplet microfluidics—A tool for single‐cell analysis

HN Joensson… - Angewandte Chemie …, 2012 - Wiley Online Library
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …

Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology

AB Theberge, F Courtois, Y Schaerli… - Angewandte Chemie …, 2010 - Wiley Online Library
Microdroplets in microfluidics offer a great number of opportunities in chemical and
biological research. They provide a compartment in which species or reactions can be …

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

F Gielen, R Hours, S Emond… - Proceedings of the …, 2016 - National Acad Sciences
Ultrahigh-throughput screening, in which members of enzyme libraries compartmentalized
in water-in-oil emulsion droplets are assayed, has emerged as a powerful format for directed …

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

JC Baret, OJ Miller, V Taly, M Ryckelynck, A El-Harrak… - Lab on a Chip, 2009 - pubs.rsc.org
We describe a highly efficient microfluidic fluorescence-activated droplet sorter (FADS)
combining many of the advantages of microtitre-plate screening and traditional fluorescence …