Ever since their introduction two decades ago, single-molecule (SM) fluorescence methods
have matured and branched out to address numerous biological questions, which were …

Intracellular cargo transport requires microtubule-based motors, kinesin and cytoplasmic
dynein, and the actin-based myosin motors to maneuver through the challenges presented …

Systems in thermodynamic equilibrium are not only characterized by time-independent
macroscopic properties, but also satisfy the principle of detailed balance in the transitions …

Cells are active systems with molecular force generation that drives complex dynamics at
the supramolecular scale. We present a quantitative study of molecular motions in cells over …

Recent developments in image processing have greatly advanced our understanding of
biomolecular processes in vitro and in vivo. In particular, using Gaussian models to fit the …

In vitro assays that reconstitute the dynamic behavior of microtubules provide insight into the
roles of microtubule-associated proteins (MAPs) in regulating the growth, shrinkage, and …

Motor proteins in the kinesin-8 family depolymerize microtubules in a length-dependent
manner that may be crucial for controlling the length of organelles such as the mitotic …

Long-distance transport in eukaryotic cells is driven by molecular motors that move along
microtubule tracks. Molecular motors of the kinesin superfamily contain a kinesin motor …

The ability to manipulate small particles of matter using the forces of light, optical trapping,
forms the basis of a number of exciting research areas, spanning fundamental physics …

Much has been learned in the past decades about molecular force generation. Single-
molecule techniques, such as atomic force microscopy, single-molecule fluorescence …