Progress in doubled haploid technology in higher plants

M Wędzony, BP Forster, I Żur, E Golemiec… - Advances in haploid …, 2009 - Springer
In the early 1990s, many basic protocols were developed for haploidy and doubled
haploidy, but most were inefficient. During the last decade, progress in technology has been …

Flow cytometry in plant breeding

SJ Ochatt - Cytometry Part A: the journal of the International …, 2008 - Wiley Online Library
Since the first report on the flow cytometric study of plant material 35 years ago, analyzing
the nuclear DNA content of field bean, an ever increasing number of applications of FCM …

Brassica biotechnology: Progress in cellular and molecular biology

V Cardoza, CN Stewart - In Vitro Cellular & Developmental Biology-Plant, 2004 - Springer
Considerable progress has been accomplished in the cellular and molecular biology of
Brassica species in the past few years. Plant regeneration has been increasingly optimized …

Ploidy level determination within the context of in vitro breeding

SJ Ochatt, EM Patat-Ochatt, A Moessner - Plant Cell, Tissue and Organ …, 2011 - Springer
Variations in genome size and chromosome complement of species provide very useful
information for biosystematic studies, and also because they influence a range of ecological …

Androgenesis-based doubled haploidy: Past, present, and future perspectives

B Hale, AMR Ferrie, S Chellamma, JP Samuel… - Frontiers in Plant …, 2022 - frontiersin.org
Androgenesis, which entails cell fate redirection within the microgametophyte, is employed
widely for genetic gain in plant breeding programs. Moreover, androgenesis-responsive …

Abiotic stress enhances androgenesis from isolated microspores of some legume species (Fabaceae)

S Ochatt, C Pech, R Grewal, C Conreux… - Journal of plant …, 2009 - Elsevier
To induce androgenesis in field pea, grass pea and the model legume species Medicago
truncatula, isolated microspores of various genotypes of these three species were submitted …

Effects of Genotype and Culture Conditions on Microspore Embryogenesis and Plant Regeneration in Brassica Rapa ssp. Rapa L.

D Shumilina, D Kornyukhin, E Domblides… - Plants, 2020 - mdpi.com
Turnip is a biennial crop and, consequently, the creation of pure lines for breeding is a time-
consuming process. The production of pure turnip lines using doubled haploids produced in …

Chromosome doubling and recovery of doubled haploid plants

KJ Kasha - Haploids in crop improvement II, 2005 - Springer
Having the ability to double the chromosome number of haploid plants has been of major
concern in their utilization for breeding, as well as for genetic, mutation and transformation …

Doubled-haploid production in chickpea (Cicer arietinum L.): role of stress treatments

RK Grewal, M Lulsdorf, J Croser, S Ochatt… - Plant cell reports, 2009 - Springer
This is the first report on the production of double-haploid chickpea embryos and
regenerated plants through anther culture using Canadian cultivar CDC Xena (kabuli) and …

Cold pretreatment enhances microspore embryogenesis in oilseed rape (Brassica napus L.)

HH Gu, P Hagberg, WJ Zhou - Plant Growth Regulation, 2004 - Springer
Stress is an essential component during embryogenesis induction in microspore culture.
Cold pretreatment has been used in cereal microspore culture but very seldom attempted in …