This review commemorates the 40th anniversary of DNA sequencing, a period in which we
have already witnessed multiple technological revolutions and a growth in scale from a few …

Determining the order of nucleic acid residues in biological samples is an integral
component of a wide variety of research applications. Over the last fifty years large numbers …

Measurement of the location of molecules in tissues is essential for understanding tissue
formation and function. Previously, we developed Slide-seq, a technology that enables …

The DNA sequencing technologies in use today produce either highly accurate short reads
or less-accurate long reads. We report the optimization of circular consensus sequencing …

Circular RNA (circRNA) is a class of noncoding RNA whose functions remain mostly
unknown. Recent studies indicate circRNA may be involved in disease pathogenesis, but …

Spatial and temporal dissection of the genomic changes occurring during the evolution of
human non–small cell lung cancer (NSCLC) may help elucidate the basis for its dismal …

A major contribution to the genome variability among individuals comes from deletions and
duplications—collectively termed copy number variations (CNVs)—which alter the diploid …

Next-generation sequencing (NGS) technologies have become increasingly available for
use in the clinical microbiology diagnostic environment. There are three main applications of …

Oxford Nanopore's third‐generation single‐molecule sequencing platform promises to
decrease costs for reagents and instrumentation. After a 2‐year hiatus following the initial …

Recent advances in sequencing technology make it possible to comprehensively catalog
genetic variation in population samples, creating a foundation for understanding human …