Exosome, a type of nanoparticles also known as small extracellular vesicles are gaining
attention as novel therapeutics for various diseases because of their ability to deliver genetic …

The HEK293 cell line has earned its place as a producer of biotherapeutics. In addition to its
ease of growth in serum-free suspension culture and its amenability to transfection, this cell …

Exosomes are small extracellular vesicles of∼ 30 to 150 nm that are secreted by all cells,
abundant in all biofluids, and play important roles in health and disease. However, details …

Biological mass spectrometry (MS) encompasses a range of methods for characterizing
proteins and other biomolecules. MS is uniquely powerful for the structural analysis of …

Mesenchymal stromal cells (MSCs) are multipotent cells obtained from many tissues
including bone marrow, adipose tissue, umbilical cord, amniotic fluid, and placenta. MSCs …

Highlights•Manufacture of biopharmaceuticals, virus-like particles and viral vectors.•System
biology,'omics, cellular phenotype and cell engineering.•Cell line development, culture and …

Serum-free cultures are preferred for application in clinical cell therapy and facilitate the
purification processes of bioproducts, such as vaccines and recombinant proteins. It can …

It is well known that surface topography plays an important role in cell behavior, including
adhesion, migration, orientation, elongation, proliferation and differentiation. Studying these …

Poor efficiency of chemotherapeutics in the eradication of Cancer Stem Cells (CSCs) has
been driving the search for more active and specific compounds. In this work, we show how …

Electron paramagnetic resonance (EPR) spectroscopy is an established technique to site-
specifically monitor conformational changes of spin-labeled biomolecules. Emerging in-cell …