Site-selective modification strategies in antibody–drug conjugates

SJ Walsh, JD Bargh, FM Dannheim… - Chemical Society …, 2021 - pubs.rsc.org
Antibody–drug conjugates (ADCs) harness the highly specific targeting capabilities of an
antibody to deliver a cytotoxic payload to specific cell types. They have garnered …

Metal-free bioorthogonal click chemistry in cancer theranostics

D Wu, K Yang, Z Zhang, Y Feng, L Rao… - Chemical Society …, 2022 - pubs.rsc.org
Bioorthogonal chemistry is a powerful tool to site-specifically activate drugs in living systems.
Bioorthogonal reactions between a pair of biologically reactive groups can rapidly and …

Contemporary Approaches for Site‐Selective Dual Functionalization of Proteins

L Xu, SL Kuan, T Weil - Angewandte Chemie International …, 2021 - Wiley Online Library
Site‐selective protein functionalization serves as an invaluable tool for investigating protein
structures and functions in complicated cellular environments and accomplishing semi …

Site‐and Stereoselective Glycomodification of Biomolecules through Carbohydrate‐Promoted Pictet–Spengler Reaction

B Liu, X Zou, Y Zhang, Y Yang, H Xu… - Angewandte …, 2024 - Wiley Online Library
Carbohydrates play pivotal roles in an array of essential biological processes and are
consequently involved in many diseases. To meet the needs of glycobiology research …

Introduction of Carbonyl Groups into Antibodies

EL Gulyak, VA Alferova, VA Korshun, KA Sapozhnikova - Molecules, 2023 - mdpi.com
Antibodies and their derivatives (scFv, Fabs, etc.) represent a unique class of biomolecules
that combine selectivity with the ability to target drug delivery. Currently, one of the most …

Single‐Step N‐Terminal Modification of Proteins via a Bio‐Inspired Copper (II)‐Mediated Aldol Reaction

K Hanaya, K Yamoto, K Taguchi… - … A European Journal, 2022 - Wiley Online Library
The chemical modification of proteins is an effective technique for manipulating the
properties and functions of proteins, and for creating protein‐based materials. The N …

Bifunctional reagents for formylglycine conjugation: pitfalls and breakthroughs

N Janson, T Krüger, L Karsten, M Boschanski… - …, 2020 - Wiley Online Library
Formylglycine‐generating enzymes specifically oxidize cysteine within the consensus
sequence CxPxR to Cα‐formylglycine (FGly). This noncanonical electrophilic amino acid …

Characterization of an N-Allylglyoxylamide-Based Bioorthogonal Nitrone Trap

D Lee, S Latour, M Emblem, HJ Clark… - Bioconjugate …, 2023 - ACS Publications
Aldehydes are attractive bioorthogonal coupling partners. The ease of manipulation of
aldehydes and their orthogonality to other classes of bioorthogonal reactions have inspired …

Enzymatic bioconjugation: a perspective from the pharmaceutical industry

A Debon, E Siirola, R Snajdrova - JACS Au, 2023 - ACS Publications
Enzymes have firmly established themselves as bespoke catalysts for small molecule
transformations in the pharmaceutical industry, from early research and development stages …

Secondary amino alcohols: traceless cleavable linkers for use in affinity capture and release

S Pomplun, CR Shugrue, AM Schmitt… - Angewandte Chemie …, 2020 - Wiley Online Library
Capture and release of peptides is often a critical operation in the pathway to discovering
materials with novel functions. However, the best methods for efficient capture impede facile …