Biomolecular phase separation: from molecular driving forces to macroscopic properties
Biological phase separation is known to be important for cellular organization, which has
recently been extended to a new class of biomolecules that form liquid-like droplets …
recently been extended to a new class of biomolecules that form liquid-like droplets …
Interaction dynamics of intrinsically disordered proteins from single-molecule spectroscopy
A Chowdhury, D Nettels… - Annual review of …, 2023 - annualreviews.org
Many proteins contain large structurally disordered regions or are entirely disordered under
physiological conditions. The functions of these intrinsically disordered proteins (IDPs) often …
physiological conditions. The functions of these intrinsically disordered proteins (IDPs) often …
Extreme dynamics in a biomolecular condensate
Proteins and nucleic acids can phase-separate in the cell to form concentrated biomolecular
condensates,,–. The functions of condensates span many length scales: they modulate …
condensates,,–. The functions of condensates span many length scales: they modulate …
Conformational ensembles of the human intrinsically disordered proteome
Intrinsically disordered proteins and regions (collectively, IDRs) are pervasive across
proteomes in all kingdoms of life, help to shape biological functions and are involved in …
proteomes in all kingdoms of life, help to shape biological functions and are involved in …
[HTML][HTML] The SARS-CoV-2 nucleocapsid protein is dynamic, disordered, and phase separates with RNA
Abstract The SARS-CoV-2 nucleocapsid (N) protein is an abundant RNA-binding protein
critical for viral genome packaging, yet the molecular details that underlie this process are …
critical for viral genome packaging, yet the molecular details that underlie this process are …
Visualizing the disordered nuclear transport machinery in situ
The approximately 120 MDa mammalian nuclear pore complex (NPC) acts as a gatekeeper
for the transport between the nucleus and cytosol. The central channel of the NPC is filled …
for the transport between the nucleus and cytosol. The central channel of the NPC is filled …
Relation between single-molecule properties and phase behavior of intrinsically disordered proteins
Proteins that undergo liquid–liquid phase separation (LLPS) have been shown to play a
critical role in many physiological functions through formation of condensed liquid-like …
critical role in many physiological functions through formation of condensed liquid-like …
Conformational ensembles of an intrinsically disordered protein consistent with NMR, SAXS, and single-molecule FRET
GNW Gomes, M Krzeminski, A Namini… - Journal of the …, 2020 - ACS Publications
Intrinsically disordered proteins (IDPs) have fluctuating heterogeneous conformations, which
makes their structural characterization challenging. Although challenging, characterization …
makes their structural characterization challenging. Although challenging, characterization …
Improved coarse‐grained model for studying sequence dependent phase separation of disordered proteins
We present improvements to the hydropathy scale (HPS) coarse‐grained (CG) model for
simulating sequence‐specific behavior of intrinsically disordered proteins (IDPs), including …
simulating sequence‐specific behavior of intrinsically disordered proteins (IDPs), including …
Global structure of the intrinsically disordered protein tau emerges from its local structure
The paradigmatic disordered protein tau plays an important role in neuronal function and
neurodegenerative diseases. To disentangle the factors controlling the balance between …
neurodegenerative diseases. To disentangle the factors controlling the balance between …