Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens

Q Liu, X Jin, J Cheng, H Zhou… - Molecular Medicine …, 2023 - spandidos-publications.com
Infectious diseases are a major global cause of morbidity and mortality, seriously affecting
public health and socioeconomic stability. Since infectious diseases can be caused by a …

Multiplexed isothermal nucleic acid amplification

O Mayboroda, I Katakis, CK O'Sullivan - Analytical biochemistry, 2018 - Elsevier
Multiplexed isothermal amplification and detection of nucleic acid sequences and
biomarkers is of increasing importance in diverse areas including advanced diagnostics …

Molecular and immunological diagnostic techniques of medical viruses

DH Reta, TS Tessema, AS Ashenef… - International Journal …, 2020 - Wiley Online Library
Viral infections are causing serious problems in human population worldwide. The recent
outbreak of coronavirus disease 2019 caused by SARS‐CoV‐2 is a perfect example how …

HCV detection, discrimination, and genotyping technologies

SD Warkad, SB Nimse, KS Song, T Kim - Sensors, 2018 - mdpi.com
According to the World Health Organization (WHO), 71 million people were living with
Hepatitis C virus (HCV) infection worldwide in 2015. Each year, about 399,000 HCV-infected …

Sensitive HIV-1 detection in a homogeneous solution based on an electrochemical molecular beacon coupled with a nafion–graphene composite film modified screen …

B Li, Z Li, B Situ, Z Dai, Q Liu, Q Wang, D Gu… - Biosensors and …, 2014 - Elsevier
A novel electrochemical sensing assay for sensitive determination of HIV-1 in a
homogeneous solution has been developed using an electrochemical molecular beacon …

[HTML][HTML] An ultrasensitive fluorescence sensing platform for HCV detection based on the T7 isothermal amplification combined with aggregation-induced emission …

W Guo, X Zhu, J Li, L Li - Sensors and Actuators Reports, 2024 - Elsevier
Hepatitis C virus infection constitutes a significant global public health concern.
Fluorescence technique (FL) is a promising biosensor, however, many conventional dyes …

Evaluation of real-time NASBA assay for the detection of SARS-CoV-2 compared with real-time PCR

V Kia, A Tafti, M Paryan… - Irish Journal of Medical …, 2023 - Springer
Abstract Purpose In January 2020, the COVID-19 pandemic started and has severely
affected all countries around the world. The clinical symptoms alone are not sufficient for a …

Unimolecular aptamer-based sensors for pathogen detection

A Green, D Ma, A Tang - US Patent 11,773,430, 2023 - Google Patents
Methods and compositions for detecting the presence of a pathogen are described. In
particular, this document provides a method of detecting a pathogen-associated nucleic acid …

Quantitative real-time PCR technique for the identification of E. coli residual DNA in streptokinase recombinant product

M Fazelahi, V Kia, H Kaghazian… - Preparative Biochemistry …, 2017 - Taylor & Francis
Recombinant streptokinase is a biopharmaceutical which is usually produced in E. coli.
Residual DNA as a contamination and risk factor may remain in the product. It is necessary …

A simple, sensitive and non-enzymatic signal amplification strategy driven by seesaw gate

W Zhang, Y Li, HW Li, Y Cheng, H Yu, Y Xie, W Yao… - Analytica Chimica …, 2020 - Elsevier
Herein, a simple enzyme-free method based on the seesaw-gate-driven isothermal signal
amplification strategy was developed for nucleic acid detection. In this method, a partially …