[HTML][HTML] Live-cell imaging in the deep learning era

JW Pylvänäinen, E Gómez-de-Mariscal… - Current Opinion in Cell …, 2023 - Elsevier
Live imaging is a powerful tool, enabling scientists to observe living organisms in real time.
In particular, when combined with fluorescence microscopy, live imaging allows the …

Light-sheets and smart microscopy, an exciting future is dawning

S Daetwyler, RP Fiolka - Communications biology, 2023 - nature.com
Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

DeLTA 2.0: A deep learning pipeline for quantifying single-cell spatial and temporal dynamics

OM O'Connor, RN Alnahhas, JB Lugagne… - PLoS computational …, 2022 - journals.plos.org
Improvements in microscopy software and hardware have dramatically increased the pace
of image acquisition, making analysis a major bottleneck in generating quantitative, single …

Live-cell imaging powered by computation

H Shroff, I Testa, F Jug, S Manley - Nature Reviews Molecular Cell …, 2024 - nature.com
The proliferation of microscopy methods for live-cell imaging offers many new possibilities
for users but can also be challenging to navigate. The prevailing challenge in live-cell …

An optogenetic toolkit for light-inducible antibiotic resistance

MB Sheets, N Tague, MJ Dunlop - Nature Communications, 2023 - nature.com
Antibiotics are a key control mechanism for synthetic biology and microbiology. Resistance
genes are used to select desired cells and regulate bacterial populations, however their use …

Advances in automated and reactive flow cytometry for synthetic biotechnology

F Delvigne, JA Martinez - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights•The accessibility to automated flow cytometry equipment has recently been
increased through the open science initiative.•Automated and reactive flow cytometry can be …

Image-guided optogenetic spatiotemporal tissue patterning using μPatternScope

S Kumar, HM Beyer, M Chen, MD Zurbriggen… - Nature …, 2024 - nature.com
In the field of tissue engineering, achieving precise spatiotemporal control over engineered
cells is critical for sculpting functional 2D cell cultures into intricate morphological shapes. In …

A light tunable differentiation system for the creation and control of consortia in yeast

C Aditya, F Bertaux, G Batt, J Ruess - Nature communications, 2021 - nature.com
Artificial microbial consortia seek to leverage division-of-labour to optimize function and
possess immense potential for bioproduction. Co-culturing approaches, the preferred mode …

Data-driven microscopy allows for automated context-specific acquisition of high-fidelity image data

O André, JK Ahnlide, N Norlin, V Swaminathan… - Cell reports …, 2023 - cell.com
Light microscopy is a powerful single-cell technique that allows for quantitative spatial
information at subcellular resolution. However, unlike flow cytometry and single-cell …

Platforms for optogenetic stimulation and feedback control

S Kumar, M Khammash - Frontiers in Bioengineering and …, 2022 - frontiersin.org
Harnessing the potential of optogenetics in biology requires methodologies from different
disciplines ranging from biology, to mechatronics engineering, to control engineering. Light …