Live imaging is a powerful tool, enabling scientists to observe living organisms in real time.
In particular, when combined with fluorescence microscopy, live imaging allows the …

Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …

The proliferation of microscopy methods for live-cell imaging offers many new possibilities
for users but can also be challenging to navigate. The prevailing challenge in live-cell …

Antibiotics are a key control mechanism for synthetic biology and microbiology. Resistance
genes are used to select desired cells and regulate bacterial populations, however their use …

Highlights•The accessibility to automated flow cytometry equipment has recently been
increased through the open science initiative.•Automated and reactive flow cytometry can be …

Light microscopy is a powerful single-cell technique that allows for quantitative spatial
information at subcellular resolution. However, unlike flow cytometry and single-cell …

Harnessing the potential of optogenetics in biology requires methodologies from different
disciplines ranging from biology, to mechatronics engineering, to control engineering. Light …

Gene expression is inherently dynamic, due to complex regulation and stochastic
biochemical events. However, the effects of these dynamics on cell phenotypes can be …

Fluorescence microscopy is essential for studying living cells, tissues and organisms.
However, the fluorescent light that switches on fluorescent molecules also harms the …

We present a computational framework to simultaneously perform image acquisition,
reconstruction, and analysis in the context of open-source microscopy automation. The …