The in vitro MN assay in 2011: origin and fate, biological significance, protocols, high throughput methodologies and toxicological relevance
M Kirsch-Volders, G Plas, A Elhajouji… - Archives of …, 2011 - Springer
Micronuclei (MN) are small, extranuclear bodies that arise in dividing cells from acentric
chromosome/chromatid fragments or whole chromosomes/chromatids lagging behind in …
chromosome/chromatid fragments or whole chromosomes/chromatids lagging behind in …
A core in vitro genotoxicity battery comprising the Ames test plus the in vitro micronucleus test is sufficient to detect rodent carcinogens and in vivo genotoxins
D Kirkland, L Reeve, D Gatehouse… - Mutation Research/Genetic …, 2011 - Elsevier
In vitro genotoxicity testing needs to include tests in both bacterial and mammalian cells, and
be able to detect gene mutations, chromosomal damage and aneuploidy. This may be …
be able to detect gene mutations, chromosomal damage and aneuploidy. This may be …
H2AX phosphorylation as a genotoxicity endpoint
The γH2AX focus assay, based on phosphorylation of the variant histone protein H2AX, was
evaluated as a genotoxicity test in immortalised wild-type mouse embryonic fibroblasts …
evaluated as a genotoxicity test in immortalised wild-type mouse embryonic fibroblasts …
Application of a new approach methodology (NAM)-based strategy for genotoxicity assessment of data-poor compounds
The conventional battery for genotoxicity testing is not well suited to assessing the large
number of chemicals needing evaluation. Traditional in vitro tests lack throughput, provide …
number of chemicals needing evaluation. Traditional in vitro tests lack throughput, provide …
A modern genotoxicity testing paradigm: Integration of the high-throughput CometChip® and the TGx-DDI transcriptomic biomarker in human HepaRG™ cell cultures
JK Buick, A Williams, MJ Meier, CD Swartz… - Frontiers in Public …, 2021 - frontiersin.org
Higher-throughput, mode-of-action-based assays provide a valuable approach to expedite
chemical evaluation for human health risk assessment. In this study, we combined the high …
chemical evaluation for human health risk assessment. In this study, we combined the high …
Evaluation of high-throughput genotoxicity assays used in profiling the US EPA ToxCast™ chemicals
Three high-throughput screening (HTS) genotoxicity assays—GreenScreen HC GADD45a-
GFP (Gentronix Ltd.), CellCiphr p53 (Cellumen Inc.) and CellSensor p53RE-bla (Invitrogen …
GFP (Gentronix Ltd.), CellCiphr p53 (Cellumen Inc.) and CellSensor p53RE-bla (Invitrogen …
The application of 3D cell models to support drug safety assessment: opportunities & challenges
A Roth, T Singer - Advanced drug delivery reviews, 2014 - Elsevier
The selection of drug candidates early in development has become increasingly important to
minimize the use of animals and to avoid costly failures of drugs later in development. In …
minimize the use of animals and to avoid costly failures of drugs later in development. In …
[HTML][HTML] The potential for complete automated scoring of the cytokinesis block micronucleus cytome assay using imaging flow cytometry
MA Rodrigues, LA Beaton-Green, RC Wilkins… - … /Genetic Toxicology and …, 2018 - Elsevier
Abstract The lymphocyte Cytokinesis-Block Micronucleus (CBMN) assay was originally
developed for the measurement of micronuclei (MN) exclusively in binucleated (BN) cells …
developed for the measurement of micronuclei (MN) exclusively in binucleated (BN) cells …
Genotoxic mode of action predictions from a multiplexed flow cytometric assay and a machine learning approach
SM Bryce, DT Bernacki, JC Bemis… - Environmental and …, 2016 - Wiley Online Library
Several endpoints associated with cellular responses to DNA damage as well as overt
cytotoxicity were multiplexed into a miniaturized,“add and read” type flow cytometric assay …
cytotoxicity were multiplexed into a miniaturized,“add and read” type flow cytometric assay …