The fitness of an individual bacterial cell is highly dependent upon the temporal tuning of
gene expression levels when subjected to different environmental cues. Kinetic regulation of …

Efficient and accurate termination is required for gene transcription in all living organisms,.
Cellular RNA polymerases in both bacteria and eukaryotes can terminate their transcription …

Genomic DNA is a crowded track where motor proteins frequently collide. It remains
underexplored whether these collisions carry physiological function. In this work, we …

Rho is a general transcription termination factor in bacteria, but many aspects of its
mechanism of action are unclear. Diverse models have been proposed for the initial …

ABSTRACT RNA polymerases are the central enzymes of gene expression and function
frequently in either a head-on or co-directional manner on the busy DNA track. Whether and …

The R-loops forming around DNA double-strand breaks (DSBs) within actively transcribed
genes play a critical role in the DSB repair process. However, the mechanisms underlying R …

RNA can regulate its own synthesis without auxiliary proteins. For example, U-rich RNA
sequences signal RNA polymerase (RNAP) to pause transcription and are required for …

Transcriptional pause is essential for all types of termination. In this single-molecule study
on bacterial Rho factor-dependent terminators, we confirm that the three Rho-dependent …

Cotranscriptional RNA folding is widely assumed to influence the timely control of gene
expression, but our understanding remains limited. In bacteria, the fluoride (F−)-sensing …

Cellular RNA polymerases (RNAPs) can become trapped on DNA or RNA, threatening
genome stability and limiting free enzyme pools, but how RNAP recycling into active states …