Serial block face scanning electron microscopy in cell biology: Applications and technology

D Smith, T Starborg - Tissue and Cell, 2019 - Elsevier
Three-dimensional electron microscopy (3DEM) is an imaging field containing several
powerful modalities such as serial section transmission electron microscopy and electron …

Quantifying Golgi structure using EM: combining volume-SEM and stereology for higher throughput

S Ferguson, AM Steyer, TM Mayhew, Y Schwab… - Histochemistry and Cell …, 2017 - Springer
Investigating organelles such as the Golgi complex depends increasingly on high-
throughput quantitative morphological analyses from multiple experimental or genetic …

[HTML][HTML] SuRVoS: super-region volume segmentation workbench

I Luengo, MC Darrow, MC Spink, Y Sun, W Dai… - Journal of Structural …, 2017 - Elsevier
Segmentation of biological volumes is a crucial step needed to fully analyse their scientific
content. Not having access to convenient tools with which to segment or annotate the data …

3D mesh processing using GAMer 2 to enable reaction-diffusion simulations in realistic cellular geometries

CT Lee, JG Laughlin… - PLoS computational …, 2020 - journals.plos.org
Recent advances in electron microscopy have enabled the imaging of single cells in 3D at
nanometer length scale resolutions. An uncharted frontier for in silico biology is the ability to …

[HTML][HTML] Impact of training data, ground truth and shape variability in the deep learning-based semantic segmentation of HeLa cells observed with electron microscopy

C Karabağ, MA Ortega-Ruíz, CC Reyes-Aldasoro - Journal of Imaging, 2023 - mdpi.com
This paper investigates the impact of the amount of training data and the shape variability on
the segmentation provided by the deep learning architecture U-Net. Further, the correctness …

A guide to analysis and reconstruction of serial block face scanning electron microscopy data

E Cocks, M Taggart, FC Rind, K White - Journal of microscopy, 2018 - Wiley Online Library
Serial block face scanning electron microscopy (SBF‐SEM) is a relatively new technique
that allows the acquisition of serially sectioned, imaged and digitally aligned ultrastructural …

Quantifying variability of manual annotation in cryo-electron tomograms

CW Hecksel, MC Darrow, W Dai… - Microscopy and …, 2016 - academic.oup.com
Although acknowledged to be variable and subjective, manual annotation of cryo-electron
tomography data is commonly used to answer structural questions and to create a “ground …

Biomass accessibility analysis using electron tomography

JD Hinkle, PN Ciesielski, K Gruchalla, KR Munch… - Biotechnology for …, 2015 - Springer
Background Substrate accessibility to catalysts has been a dominant theme in theories of
biomass deconstruction. However, current methods of quantifying accessibility do not …

Image segmentation and analysis for densification mapping of nanoporous gold after nanoindentation

C Richert, Y Wu, M Hablitzel, ET Lilleodden, N Huber - MRS advances, 2021 - Springer
Segmentation of scanning electron microscopy (SEM) images of focused ion beam (FIB)
cross-sections through indented regions in nanoporous gold (np-Au) is carried out. A key …

Volume segmentation and analysis of biological materials using SuRVoS (Super-region Volume Segmentation) Workbench

MC Darrow, I Luengo, M Basham… - Journal of …, 2017 - pmc.ncbi.nlm.nih.gov
Segmentation is the process of isolating specific regions or objects within an imaged
volume, so that further study can be undertaken on these areas of interest. When …