Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

Organoids are three-dimensional (3D) miniature structures cultured in vitro produced from
either human pluripotent stem cells (hPSCs) or adult stem cells (AdSCs) derived from …

The mutational landscape of colorectal cancer (CRC) does not enable predictions to be
made about the survival of patients or their response to therapy. Instead, studying the …

Organoids are in vitro miniaturized and simplified model systems of organs that have gained
enormous interest for modelling tissue development and disease, and for personalized …

The historical reliance of biological research on the use of animal models has sometimes
made it challenging to address questions that are specific to the understanding of human …

Colorectal cancer has served as a genetic and biological paradigm for the evolution of solid
tumors, and these insights have illuminated early detection, risk stratification, prevention …

Adult stem cell–based organoid technology is a versatile tool for the generation and long-
term maintenance of near-native 3D epithelial tissues in vitro. The generation of cancer …

Abstract Around 30–40% of patients with colorectal cancer (CRC) undergoing curative
resection of the primary tumour will develop metastases in the subsequent years. Therapies …

Simple Summary Cell cultures can be carried out in three dimensions (3D). Organoids and
spheroids are different 3D cell culture models that can be cultured with different techniques …

Mutation-induced activation of WNT–β-catenin signalling is a frequent driver event in human
cancer. Sustained WNT–β-catenin pathway activation endows cancer cells with sustained …