In a clinical assay, enzymes are essential biomarkers for human disease diagnosis. In this
work, a spectral-resolved single-particle detection (SPD) method is introduced to quantify …

This mini-review discusses the evolution of fluorescence as a tool to study living cells and
tissues in vitro and the present role of fluorescent protein biosensors (FPBs) in …

Highlights•Knowledge about intracellular kinetics can be obtained without synchronization.•
Single molecule sensitivity enables studies of processes involving low copy number …

Nanomedicine is the application of nanotechnology in treatment, diagnosis, monitoring and
control of biological systems, and is at the leading-edge of clinical medicine and preclinical …

Single-cell methods are a powerful application in microbial research to study the molecular
mechanism underlying phenotypic heterogeneity and cell-to-cell variability. Here, we …

Light microscopy has emerged as one of the fundamental methods to analyze biological
systems; novel techniques of 3D microscopy and super-resolution microscopy (SRM) with …

Fluorescent probes with larger Stokes shifts in the far-visible and near-infrared spectral
region (600–900 nm) are more superior for cellular imaging and biological analysis due to …

An anti-galvanic reduction method using [Ag62S13 (SBut) 32] 4+ nanoclusters as the metal
ion probe for detecting Cu2+ is reported here. We found [Ag62S13 (SBut) 32] 4+ can reduce …

Single-molecule fluorescence imaging with living cells offers a new approach to visualize
and monitor individual proteins during their cellular activities. It facilities the study of cell …

Long-term holding and precise handling of growing plant tissues during in vitro cultivation
has been a major hurdle for experimental studies related to plant development and …