CRISPR/Cas system: recent advances and future prospects for genome editing

H Manghwar, K Lindsey, X Zhang, S Jin - Trends in plant science, 2019 - cell.com
Genome editing (GE) has revolutionized biological research through the new ability to
precisely edit the genomes of living organisms. In recent years, various GE tools have been …

Molecular farming–the slope of enlightenment

R Fischer, JF Buyel - Biotechnology advances, 2020 - Elsevier
Molecular farming can be defined as the use of plants to produce recombinant protein
products. The technology is now> 30 years old. The early promise of molecular farming was …

Plant gene editing through de novo induction of meristems

MF Maher, RA Nasti, M Vollbrecht, CG Starker… - Nature …, 2020 - nature.com
Plant gene editing is typically performed by delivering reagents such as Cas9 and single
guide RNAs to explants in culture. Edited cells are then induced to differentiate into whole …

Phytophthora sojae boosts host trehalose accumulation to acquire carbon and initiate infection

X Zhu, D Fang, D Li, J Zhang, H Jiang, L Guo… - Nature …, 2023 - nature.com
Successful infection by pathogenic microbes requires effective acquisition of nutrients from
their hosts. Root and stem rot caused by Phytophthora sojae is one of the most important …

Plant-based biopharmaceutical engineering

L Eidenberger, B Kogelmann… - Nature Reviews …, 2023 - nature.com
Plants can be engineered to recombinantly produce high-quality proteins such as
therapeutic proteins and vaccines, also known as molecular farming. Molecular farming can …

CRISPR-mediated engineering across the central dogma in plant biology for basic research and crop improvement

D Pramanik, RM Shelake, MJ Kim, JY Kim - Molecular Plant, 2021 - cell.com
The central dogma (CD) of molecular biology is the transfer of genetic information from DNA
to RNA to protein. Major CD processes governing genetic flow include the cell cycle, DNA …

Plant molecular farming–integration and exploitation of side streams to achieve sustainable biomanufacturing

JF Buyel - Frontiers in plant science, 2019 - frontiersin.org
Plants have unique advantages over other systems such as mammalian cells for the
production of valuable small molecules and proteins. The benefits cited most often include …

[HTML][HTML] On the verge of the market–Plant factories for the automated and standardized production of biopharmaceuticals

JW Huebbers, JF Buyel - Biotechnology Advances, 2021 - Elsevier
The market for biopharmaceuticals is dominated by recombinant proteins and is driven
mainly by the development of vaccines and antibodies. Manufacturing predominantly relies …

Plant glycosides and glycosidases: a treasure-trove for therapeutics

K Kytidou, M Artola, HS Overkleeft… - Frontiers in plant …, 2020 - frontiersin.org
Plants contain numerous glycoconjugates that are metabolized by specific
glucosyltransferases and hydrolyzed by specific glycosidases, some also catalyzing …

[HTML][HTML] Plant glycoengineering for designing next-generation vaccines and therapeutic proteins

R Strasser - Biotechnology Advances, 2023 - Elsevier
Protein glycosylation has a huge impact on biological processes in all domains of life. The
type of glycan present on a recombinant glycoprotein depends on protein intrinsic features …