Protein assembly by design

J Zhu, N Avakyan, A Kakkis, AM Hoffnagle… - Chemical …, 2021 - ACS Publications
Proteins are nature's primary building blocks for the construction of sophisticated molecular
machines and dynamic materials, ranging from protein complexes such as photosystem II …

Protein cages: from fundamentals to advanced applications

TGW Edwardson, MD Levasseur, S Tetter… - Chemical …, 2022 - ACS Publications
Proteins that self-assemble into polyhedral shell-like structures are useful molecular
containers both in nature and in the laboratory. Here we review efforts to repurpose diverse …

Precise assembly of proteins and carbohydrates for next-generation biomaterials

L Li, G Chen - Journal of the American Chemical Society, 2022 - ACS Publications
The complexity and diversity of biomacromolecules make them a unique class of building
blocks for generating precise assemblies. They are particularly available to a new …

Encapsulin cargo loading: progress and potential

JA Jones, R Benisch, TW Giessen - Journal of Materials Chemistry B, 2023 - pubs.rsc.org
Encapsulins are a recently discovered class of prokaryotic self-assembling icosahedral
protein nanocompartments measuring between 24 and 42 nm in diameter, capable of …

[HTML][HTML] Protein scaffolds: a tool for multi-enzyme assembly

S Gad, S Ayakar - Biotechnology Reports, 2021 - Elsevier
The synthesis of complex molecules using multiple enzymes simultaneously in one reaction
vessel has rapidly emerged as a new frontier in the field of bioprocess technology. However …

Encapsulin nanocontainers as versatile scaffolds for the development of artificial metabolons

MC Jenkins, S Lutz - ACS Synthetic Biology, 2021 - ACS Publications
The construction of non-native biosynthetic pathways represents a powerful, modular
strategy for the production of valuable synthons and fine chemicals. Accordingly, artificially …

Artificial organelles: towards adding or restoring intracellular activity

RAJF Oerlemans, SBPE Timmermans… - …, 2021 - Wiley Online Library
Compartmentalization is one of the main characteristics that define living systems. Creating
a physically separated microenvironment allows nature a better control over biological …

Load and display: engineering encapsulin as a modular nanoplatform for protein-cargo encapsulation and protein-ligand decoration using split intein and SpyTag …

H Choi, S Eom, H Kim, Y Bae, HS Jung… - …, 2021 - ACS Publications
Protein cage nanoparticles have a unique spherical hollow structure that provides a
modifiable interior space and an exterior surface. For full application, it is desirable to utilize …

Design of Ligand-Operable Protein-Cages That Open Upon Specific Protein Binding

EJ Lee, N Gladkov, JE Miller, TO Yeates - ACS Synthetic Biology, 2023 - ACS Publications
Protein nanocages have diverse applications in medicine and biotechnology, including
molecular delivery. However, although numerous studies have demonstrated the ability of …

Designing protease-triggered protein cages

JE Miller, Y Srinivasan, NP Dharmaraj… - Journal of the …, 2022 - ACS Publications
Proteins that self-assemble into enclosed polyhedral cages, both naturally and by design,
are garnering attention for their prospective utility in the fields of medicine and …