In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Nuclear magnetic resonance (NMR) spectroscopy is a well-established method for
analyzing protein structure, interaction, and dynamics at atomic resolution and in various …

A detailed knowledge of the complex processes that make cells and organisms alive is
fundamental in order to understand diseases and to develop novel drugs and therapeutic …

Protein–ligand interactions are of fundamental importance in almost all processes in living
organisms. The ligands comprise small molecules, drugs or biological macromolecules and …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

In recent years, better instrumentation and greater computing power have enabled the
imaging of elusive biomolecule dynamics in cells, driving many advances in understanding …

Cellular function is generally depicted at the level of functional pathways and detailed
structural mechanisms, based on the identification of specific protein–protein interactions …

Classical structural biology approaches allow structural characterization of biological
macromolecules in vitro, far from their physiological context. Nowadays, thanks to the wealth …

Most knowledge of protein structure and function is derived from experiments performed with
purified protein resuspended in dilute, buffered solutions. However, proteins function in the …

How proteins sense and navigate the cellular interior to find their functional partners remains
poorly understood. An intriguing aspect of this search is that it relies on diffusive encounters …