Although they are often exploited to facilitate the expression and purification of recombinant
proteins, every affinity tag, whether large or small, has the potential to interfere with the …

Proteolytic enzymes (also termed peptidases, proteases and proteinases) are capable of
hydrolyzing peptide bonds in proteins. They can be found in all living organisms, from …

Corynebacterium glutamicum was metabolically engineered for the production of glutaric
acid, a C5 dicarboxylic acid that can be used as platform building block chemical for nylons …

The production of extracellular lipases by staphylococcal species has been known for many
years [1, 2]. Interest in these lipases was originally stimulated by observations that certain …

Immobilized metal affinity chromatography (IMAC) of proteins containing poly-histidine
fusion tags is an efficient research tool for purifying recombinant proteins from crude cellular …

The sapN gene, encoding the extracellular subtilisin SAPN, a serine alkaline protease from
Melghiribacillus thermohalophilus Nari2A T, was isolated, sequenced, and heterologously …

The ability of a non-commercial immobilized Staphylococcus aureus lipase to catalyze the
esterification of eugenol with benzoic acid was checked and the antioxidant power of the …

Specific tags with defined amino acid residues are widely used to purify or probe target
proteins. Interestingly, the tagging system occasionally results in an increase of the …

Recombinant proteins were often expressed with His-tag to simplify the purification process.
Among them, transaminase was mostly expressed with fusion tags and widely used in the …

Polyhydroxyalkanoates (PHAs) are carbon and energy storage polymers produced by a
variety of microbial organisms under nutrient-limited conditions. They have been considered …