An overview of the recent advances in cryo-electron microscopy for life sciences
Cryo-electron microscopy (CryoEM) has superseded X-ray crystallography and NMR to
emerge as a popular and effective tool for structure determination in recent times. It has …
emerge as a popular and effective tool for structure determination in recent times. It has …
Cryo-electron tomography related radiation-damage parameters for individual-molecule 3D structure determination
To understand the dynamic structure–function relationship of soft-and biomolecules, the
determination of the three-dimensional (3D) structure of each individual molecule …
determination of the three-dimensional (3D) structure of each individual molecule …
[HTML][HTML] Sphericity and roundness computation for particles using the extreme vertices model
Shape is a property studied for many kinds of particles. Among shape parameters, sphericity
and roundness indices had been largely studied to understand several processes. Some of …
and roundness indices had been largely studied to understand several processes. Some of …
LoTToR: an algorithm for missing-wedge correction of the low-tilt tomographic 3D reconstruction of a single-molecule structure
A single-molecule three-dimensional (3D) structure is essential for understanding the
thermal vibrations and dynamics as well as the conformational changes during the chemical …
thermal vibrations and dynamics as well as the conformational changes during the chemical …
MBIR: A cryo-ET 3D reconstruction method that effectively minimizes missing wedge artifacts and restores missing information
Abstract Cryo-Electron Tomography (cryo-ET) has become an essential technique in
revealing cellular and macromolecular assembly structures in their native states. However …
revealing cellular and macromolecular assembly structures in their native states. However …
Three-dimensional structural dynamics of DNA origami Bennett linkages using individual-particle electron tomography
Scaffolded DNA origami has proven to be a powerful and efficient technique to fabricate
functional nanomachines by programming the folding of a single-stranded DNA template …
functional nanomachines by programming the folding of a single-stranded DNA template …
The unremarkable alveolar epithelial glycocalyx: a thorium dioxide-based electron microscopic comparison after heparinase or pneumolysin treatment
S Timm, M Lettau, J Hegermann, ML Rocha… - Histochemistry and Cell …, 2023 - Springer
Recent investigations analyzed in depth the biochemical and biophysical properties of the
endothelial glycocalyx. In comparison, this complex cell-covering structure is largely …
endothelial glycocalyx. In comparison, this complex cell-covering structure is largely …
Missing wedge completion via unsupervised learning with coordinate networks
Cryogenic electron tomography (cryoET) is a powerful tool in structural biology, enabling
detailed 3D imaging of biological specimens at a resolution of nanometers. Despite its …
detailed 3D imaging of biological specimens at a resolution of nanometers. Despite its …
Protocols for Subtomogram Averaging of Membrane Proteins in the Dynamo Software Package
PP Navarro, H Stahlberg… - Frontiers in molecular …, 2018 - frontiersin.org
Cryo-electron tomography allows low-resolution three-dimensional (3D) viewing of cellular
organelles and macromolecular complexes present as multiple copies within a tomogram …
organelles and macromolecular complexes present as multiple copies within a tomogram …
A Monte Carlo framework for missing wedge restoration and noise removal in cryo-electron tomography
E Moebel, C Kervrann - Journal of Structural Biology: X, 2020 - Elsevier
We propose a statistical method to address an important issue in cryo-electron tomography
image analysis: reduction of a high amount of noise and artifacts due to the presence of a …
image analysis: reduction of a high amount of noise and artifacts due to the presence of a …