Achieving controlled biomolecule–biomaterial conjugation

CD Spicer, ET Pashuck, MM Stevens - Chemical reviews, 2018 - ACS Publications
The conjugation of biomolecules can impart materials with the bioactivity necessary to
modulate specific cell behaviors. While the biological roles of particular polypeptide …

[HTML][HTML] Making sense of “nonsense” and more: Challenges and opportunities in the genetic code expansion, in the world of tRNA modifications

OM Lateef, MO Akintubosun, OT Olaoba… - International Journal of …, 2022 - mdpi.com
The evolutional development of the RNA translation process that leads to protein synthesis
based on naturally occurring amino acids has its continuation via synthetic biology, the so …

[图书][B] Life in the Universe

D Schulze-Makuch, LN Irwin - 2004 - Springer
While grounded in the hard sciences of physics, chemistry, geology, and astronomy, as well
as well-accepted principles in the life sciences, astrobiology remains the most speculative of …

A visible and near-infrared light activatable diazocoumarin probe for fluorogenic protein labeling in living cells

SY Dai, D Yang - Journal of the American Chemical Society, 2020 - ACS Publications
Chemical modification of proteins in living cells permits valuable glimpses into the molecular
interactions that underpin dynamic cellular events. While genetic engineering methods are …

[HTML][HTML] The optimality of the standard genetic code assessed by an eight-objective evolutionary algorithm

M Wnętrzak, P Błażej, D Mackiewicz… - BMC Evolutionary …, 2018 - Springer
Background The standard genetic code (SGC) is a unique set of rules which assign amino
acids to codons. Similar amino acids tend to have similar codons indicating that the code …

Engineering translation components for genetic code expansion

S Kim, H Yi, YT Kim, HS Lee - Journal of Molecular Biology, 2022 - Elsevier
The expansion of the genetic code consisting of four bases and 20 amino acids into diverse
building blocks has been an exciting topic in synthetic biology. Many biochemical …

[HTML][HTML] Enhancing the performance of a mutant pyrrolysyl-tRNA synthetase to create a highly versatile eukaryotic cell-free protein synthesis tool

JL Schloßhauer, A Zemella, SK Dondapati… - Scientific Reports, 2023 - nature.com
Modification of proteins with a broad range of chemical functionalities enables the
investigation of protein structure and activity by manipulating polypeptides at single amino …

[HTML][HTML] Cell engineering and cultivation of Chinese hamster ovary cells for the development of orthogonal eukaryotic cell-free translation systems

JL Schloßhauer, N Cavak, A Zemella… - Frontiers in Molecular …, 2022 - frontiersin.org
The investigation of protein structures, functions and interactions often requires
modifications to adapt protein properties to the specific application. Among many possible …

Synthetic alienation of microbial organisms by using genetic code engineering: Why and how?

V Kubyshkin, N Budisa - Biotechnology journal, 2017 - Wiley Online Library
The main goal of synthetic biology (SB) is the creation of biodiversity applicable for
biotechnological needs, while xenobiology (XB) aims to expand the framework of natural …

Solid-phase peptide capture and release for bulk and single-molecule proteomics

CJ Howard, BM Floyd, AM Bardo… - ACS chemical …, 2020 - ACS Publications
The field of proteomics has expanded recently with more sensitive techniques for the bulk
measurement of peptides as well as single-molecule techniques. One limiting factor for …