The molecular analysis of genetic diseases relies on several technical approaches which
allow genetic and physical mapping, characterization of the gene structure, expression …

Genotypic selection enriches a particular DNA sequence relative to another closely-related
DNA sequence based only on a change of one or a few bases. This review is a survey of the …

The human lymphoblastoid MT1 B-cell line was previously isolated as one of a series of
mutant cells able to survive the cytotoxic effects of N-methyl-N'-nitro-N-nitrosoguanidine …

The K-ras mutation is one of the most common genetic alterations found in human lung
cancer. To evaluate the prognostic value of ras gene alterations in lung cancer in a US …

DNA synthesis fidelities of two thermostable DNA polymerases, Thermus aquaticus (Taq)
and Thermococcus litoralis (Tli, also known as VentTM) and a non-thermostable enzyme, a …

Publisher Summary This chapter presents the study of conformational transitions in nucleic
acids using denaturing gradient gel electrophoresis (DGGE). The term melting is used to …

Age-specific cancer rates show large historical increases that indict environmental risk
factors. But these environmental factors did not necessarily act by increasing oncomutation …

Blood is the obvious tissue of choice for monitoring DNA damage in the human population,
as it is the most easily obtained tissue. Several genetic endpoints are used to measure …

We examined the toxicity, mutagenicity, and mutational spectra of N-ethyl-N-nitrosourea
(ENU) in three Epstein-Barr virus-transformed human lymphoblastoid cell lines, each with a …

We have determined the mutational spectrum of N-ethyl-N-nitrosourea (ENU) in exon 3 of
the hypoxanthine (guanine) phosphoribosyltransferase gene (Hprt) in splenic T cells …