Single-cell omics: experimental workflow, data analyses and applications

F Sun, H Li, D Sun, S Fu, L Gu, X Shao, Q Wang… - Science China Life …, 2024 - Springer
Cells are the fundamental units of biological systems and exhibit unique development
trajectories and molecular features. Our exploration of how the genomes orchestrate the …

Opportunities and challenges in the application of single-cell and spatial transcriptomics in plants

C Chen, Y Ge, L Lu - Frontiers in Plant Science, 2023 - frontiersin.org
Single-cell and spatial transcriptomics have diverted researchers' attention from the
multicellular level to the single-cell level and spatial information. Single-cell transcriptomes …

Single-nuclei transcriptome analysis of the shoot apex vascular system differentiation in Populus

D Conde, PM Triozzi, WJ Pereira, HW Schmidt… - …, 2022 - journals.biologists.com
Differentiation of stem cells in the plant apex gives rise to aerial tissues and organs.
Presently, we lack a lineage map of the shoot apex cells in woody perennials–a crucial gap …

Transcription factor PagMYB31 positively regulates cambium activity and negatively regulates xylem development in poplar

Y Zhang, S Chen, L Xu, S Chu, X Yan, L Lin… - The Plant …, 2024 - academic.oup.com
Wood formation involves consecutive developmental steps, including cell division of
vascular cambium, xylem cell expansion, secondary cell wall (SCW) deposition, and …

[HTML][HTML] Current status and trends in forest genomics

D Borthakur, V Busov, XH Cao, Q Du, O Gailing… - Forestry …, 2022 - maxapress.com
Forests are not only the most predominant of the Earth's terrestrial ecosystems, but are also
the core supply for essential products for human use. However, global climate change and …

Single-cell transcriptomics is revolutionizing the improvement of plant biotechnology research: recent advances and future opportunities

H Kaur, P Jha, SJ Ochatt, V Kumar - Critical Reviews in …, 2024 - Taylor & Francis
Single-cell approaches are a promising way to obtain high-resolution transcriptomics data
and have the potential to revolutionize the study of plant growth and development. Recent …

Development of High-Quality Nuclei Isolation to Study Plant Root–Microbe Interaction for Single-Nuclei Transcriptomic Sequencing in Soybean

LW D'Agostino, L Yong-Villalobos, L Herrera-Estrella… - Plants, 2023 - mdpi.com
Single-nucleus RNA sequencing (sNucRNA-seq) is an emerging technology that has been
rapidly adopted and demonstrated to be a powerful tool for detailed characterization of each …

[HTML][HTML] The single-cell transcriptome program of nodule development cellular lineages in Medicago truncatula

WJ Pereira, J Boyd, D Conde, PM Triozzi, KM Balmant… - Cell Reports, 2024 - cell.com
Legumes establish a symbiotic relationship with nitrogen-fixing rhizobia by developing
nodules. Nodules are modified lateral roots that undergo changes in their cellular …

An optimized FACS-free single-nucleus RNA sequencing (snRNA-seq) method for plant science research

K Wang, C Zhao, S Xiang, K Duan, X Chen, X Guo… - Plant Science, 2023 - Elsevier
Recently, single-cell RNA sequencing (scRNA-seq) provides unprecedented power for
accurately understanding gene expression regulatory mechanisms. However, scRNA-seq …

Single nucleus RNA-sequencing: how it's done, applications and limitations

J Fischer, T Ayers - Emerging Topics in Life Sciences, 2021 - portlandpress.com
Single nuclei RNA-sequencing (sNuc-Seq) is a methodology which uses isolated nuclei
instead of whole cells to profile gene expression. By using droplet microfluidic technologies …