Single-molecule techniques have advanced our understanding of transcription by RNA
polymerase (RNAP). A new arsenal of approaches, including single-molecule fluorescence …

The kinetics and mechanisms of transcription are now being investigated by a repertoire of
single-molecule techniques, including optical and magnetic tweezers, high-sensitivity …

Atomic force microscopy (AFM) has been used to image, at single molecule resolution,
transcription events by Escherichia coli RNA polymerase (RNAP) on a linear DNA template …

Atomic force microscopy (AFM) has made significant contributions to the study of protein-
DNA interactions by making it possible to topographically image biological samples. A …

Cell-to-cell variability plays a critical role in cellular responses and decision-making in a
population, and transcriptional bursting has been broadly studied by experimental and …

Recent advances in atomic force microscopy (AFM) imaging of nucleic acids include the
visualization of DNA and RNA incorporated into devices and patterns, and into structures …

Archaea are prokaryotes with a single DNA-dependent RNA polymerase (RNAP) that is
homologous to, and likely resembles the ancestor of all three eukaryotic RNAPs. In vitro …

The retention of transcription proteins at an actively transcribed gene contributes to
maintenance of the active transcriptional state and increases the rate of subsequent …

Eukaryotic RNA polymerase (Pol) III is recruited to target promoters by a stable preinitiation
complex containing transcription factors TFIIIC and TFIIIB. After the first transcription cycle …

Gene transcription is repetitive, enabling the synthesis of multiple copies of identical RNA
molecules from the same template. The cyclic process of RNA synthesis from active genes …