Mycobacterium avium subspecies paratuberculosis (MAP) is a member of the M avium
complex (MAC). It differs genetically from other MAC in having 14 to 18 copies of IS900 and …

Brucellosis is a zoonosis caused by facultative intracellular bacteria of the genus Brucella,
which are widely distributed in both humans and animals, especially in the developing …

Culture is considered as the reference standard assay for diagnosis of Brucella spp. in
humans and animals but it is time-consuming and hazardous. In this study, we evaluated the …

Human brucellosis poses a significant public health problem in many developing countries
and requires fast and accurate diagnosis. A PCR assay amplifying part of the 31-kDa …

In the diagnosis of human brucellosis, PCR could be a more sensitive technique than blood
cultures and more specific than conventional serological tests. We compared three different …

Polymerase chain reaction (PCR) is an in vitro technique for the nucleic acid amplification,
which is commonly used to diagnose infectious diseases. The use of PCR for pathogens …

The only unequivocal method for diagnosing sheep and goats infection due to Brucella
melitensis is bacterial isolation on appropriate culture media (ie the Farrell and modified …

This study aimed at developing a real-time polymerase chain reaction (PCR) assay for the
rapid diagnosis of human brucellosis on clinical specimens. Three assays with hybridization …

Brucellosis is a widespread zoonosis. Currently the diagnosis of this zoonosis is based on
microbiological and serological laboratory tests. Polymerase chain reaction (PCR) has been …

A pair of primers directed to 16S-23S rDNA interspacer (ITS) was designed directed to
Brucella genetic sequences in order to develop a polymerase chain reaction (PCR) …