When tissue antigens and antibodies get along: revisiting the technical aspects of immunohistochemistry—the red, brown, and blue technique

JA Ramos-Vara, MA Miller - Veterinary pathology, 2014 - journals.sagepub.com
Once focused mainly on the characterization of neoplasms, immunohistochemistry (IHC)
today is used in the investigation of a broad range of disease processes with applications in …

Technical aspects of immunohistochemistry

JA Ramos-Vara - Veterinary pathology, 2005 - journals.sagepub.com
Immunohistochemistry is an integral technique in many veterinary laboratories for diagnostic
and research purposes. In the last decade, the ability to detect antigens (Ags) in tissue …

Expression of specific chemokines and chemokine receptors in the central nervous system of multiple sclerosis patients

TL Sørensen, M Tani, J Jensen… - The Journal of …, 1999 - Am Soc Clin Investig
Chemokines direct tissue invasion by specific leukocyte populations. Thus, chemokines may
play a role in multiple sclerosis (MS), an idiopathic disorder in which the central nervous …

Effect of formalin tissue fixation and processing on immunohistochemistry

M Werner, A Chott, A Fabiano… - The American journal of …, 2000 - journals.lww.com
Although immunohistochemistry is routinely performed by many pathology laboratories, its
standardization still lags behind. A major cause of variation in the reproducibility of …

Antigen retrieval immunohistochemistry: past, present, and future

SR Shi, RJ Cote, CR Taylor - Journal of Histochemistry & …, 1997 - journals.sagepub.com
The antigen retrieval (AR) technique, which is predominantly based on high-temperature
heating of tissues, is used as a non-enzymatic pretreatment for immunohistochemical …

Antigen retrieval techniques: current perspectives

SR Shi, RJ Cote, CR Taylor - Journal of Histochemistry & …, 2001 - journals.sagepub.com
Development of the antigen retrieval (AR) technique, a simple method of boiling archival
paraffin-embedded tissue sections in water to enhance the signal of immunohistochemistry …

Loss of estrogen receptor in recurrent breast cancer is associated with poor response to endocrine therapy.

T Kuukasjärvi, J Kononen, H Helin, K Holli… - Journal of Clinical …, 1996 - ascopubs.org
PURPOSE Up to 30% to 40% of metastases from hormone receptor-positive primary breast
cancer do not respond to endocrine therapy. We studied how often hormone receptor status …

The EnVision++ system: a new immunohistochemical method for diagnostics and research. Critical comparison with the APAAP, ChemMate, CSA, LABC, and SABC …

E Sabattini, K Bisgaard, S Ascani, S Poggi… - Journal of clinical …, 1998 - jcp.bmj.com
AIM: To assess a newly developed immunohistochemical detection system, the EnVision++.
METHODS: A large series of differently processed normal and pathological samples and 53 …

Antigen retrieval techniques in immunohistochemistry: comparison of different methods

SA Pileri, G Roncador, C Ceccarelli… - The Journal of …, 1997 - Wiley Online Library
Routine sections of normal and pathological samples fixed in 10 per cent buffered formalin
or B5, including EDTA‐decalcified bone‐marrow biopsies, were tested with 61 antibodies …

Reducing soft-tissue shrinkage artefacts caused by staining with Lugol's solution

Y Dawood, J Hagoort, BA Siadari, JM Ruijter… - Scientific reports, 2021 - nature.com
Diffusible iodine-based contrast-enhanced computed tomography (diceCT) is progressively
used in clinical and morphological research to study developmental anatomy. Lugol's …