Proteomic challenges: sample preparation techniques for microgram-quantity protein analysis from biological samples

P Feist, AB Hummon - International journal of molecular sciences, 2015 - mdpi.com
Proteins regulate many cellular functions and analyzing the presence and abundance of
proteins in biological samples are central focuses in proteomics. The discovery and …

Abiotic stress responses in plant roots: a proteomics perspective

D Ghosh, J Xu - Frontiers in plant science, 2014 - frontiersin.org
Abiotic stress conditions adversely affect plant growth, resulting in significant decline in crop
productivity. To mitigate and recover from the damaging effects of such adverse …

Multi-omics analysis reveals differential molecular responses to cadmium toxicity in rice root tip and mature zone

L Kuang, T Yan, F Gao, W Tang, D Wu - Journal of Hazardous Materials, 2024 - Elsevier
Cadmium (Cd) is a highly toxic heavy metal that can be readily absorbed by plants and
enriched in human body. Rice (Oryza sativa L.) yield and grain quality are affected by …

Systems biology of tomato fruit development: combined transcript, protein, and metabolite analysis of tomato transcription factor (nor, rin) and ethylene receptor (Nr) …

S Osorio, R Alba, CMB Damasceno… - Plant …, 2011 - academic.oup.com
Tomato (Solanum lycopersicum) is an established model to study fleshy fruit development
and ripening. Tomato ripening is regulated independently and cooperatively by ethylene …

Plant leaf proteins for food applications: Opportunities and challenges

AA Anoop, PKS Pillai, M Nickerson… - … Reviews in Food …, 2023 - Wiley Online Library
Plant‐based proteins are gaining a lot of attention for their health benefits and are
considered as an alternative to animal proteins for developing sustainable food systems …

Oxidative stress and heavy metals in plants

R Fryzova, M Pohanka, P Martinkova… - … and toxicology volume …, 2018 - Springer
Oxidative stress is a pathological process related to not only animal kingdom but also plants.
Regarding oxidative stress in plants, heavy metals are frequently discussed as causative …

Treatment of Common Sunflower (Helianthus annus L.) Seeds with Radio-frequency Electromagnetic Field and Cold Plasma Induces Changes in Seed …

V Mildažienė, V Aleknavičiūtė, R Žūkienė, G Paužaitė… - Scientific reports, 2019 - nature.com
Abstract Treatment of plant seeds with electromagnetic fields or non-thermal plasmas aims
to take advantage of plant functional plasticity towards stimulation of plant agricultural …

MPLEx: a robust and universal protocol for single-sample integrative proteomic, metabolomic, and lipidomic analyses

ES Nakayasu, CD Nicora, AC Sims… - …, 2016 - Am Soc Microbiol
Integrative multi-omics analyses can empower more effective investigation and complete
understanding of complex biological systems. Despite recent advances in a range of omics …

Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis

X Wu, E Xiong, W Wang, M Scali, M Cresti - Nature protocols, 2014 - nature.com
Crop plants contain large amounts of secondary compounds that interfere with protein
extraction and gel-based proteomic analysis. Thus, a protein extraction protocol that can be …

Proteins of diverse function and subcellular location are lysine acetylated in Arabidopsis

I Finkemeier, M Laxa, L Miguet, AJM Howden… - Plant …, 2011 - academic.oup.com
Acetylation of the ε-amino group of lysine (Lys) is a reversible posttranslational modification
recently discovered to be widespread, occurring on proteins outside the nucleus, in most …