Solid-state NMR: Methods for biological solids

S Ahlawat, KR Mote, NA Lakomek… - Chemical Reviews, 2022 - ACS Publications
In the last two decades, solid-state nuclear magnetic resonance (ssNMR) spectroscopy has
transformed from a spectroscopic technique investigating small molecules and industrial …

Structure of FUS protein fibrils and its relevance to self-assembly and phase separation of low-complexity domains

DT Murray, M Kato, Y Lin, KR Thurber, I Hung… - Cell, 2017 - cell.com
Polymerization and phase separation of proteins containing low-complexity (LC) domains
are important factors in gene expression, mRNA processing and trafficking, and localization …

Fused in sarcoma: properties, self-assembly and correlation with neurodegenerative diseases

C Chen, X Ding, N Akram, S Xue, SZ Luo - Molecules, 2019 - mdpi.com
Fused in sarcoma (FUS) is a DNA/RNA binding protein that is involved in RNA metabolism
and DNA repair. Numerous reports have demonstrated by pathological and genetic analysis …

[HTML][HTML] Molecular structure of β-amyloid fibrils in Alzheimer's disease brain tissue

JX Lu, W Qiang, WM Yau, CD Schwieters, SC Meredith… - Cell, 2013 - cell.com
In vitro, β-amyloid (Aβ) peptides form polymorphic fibrils, with molecular structures that
depend on growth conditions, plus various oligomeric and protofibrillar aggregates. Here …

Rapid proton-detected NMR assignment for proteins with fast magic angle spinning

E Barbet-Massin, AJ Pell, JS Retel… - Journal of the …, 2014 - ACS Publications
Using a set of six 1H-detected triple-resonance NMR experiments, we establish a method for
sequence-specific backbone resonance assignment of magic angle spinning (MAS) nuclear …

Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning

P Fricke, V Chevelkov, M Zinke, K Giller, S Becker… - Nature …, 2017 - nature.com
Solid-state NMR (ssNMR) is a technique that allows the study of protein structure and
dynamics at atomic detail. In contrast to X-ray crystallography and cryo-electron microscopy …

Structural characterization of the D290V mutation site in hnRNPA2 low-complexity–domain polymers

DT Murray, X Zhou, M Kato, S Xiang… - Proceedings of the …, 2018 - National Acad Sciences
Human genetic studies have given evidence of familial, disease-causing mutations in the
analogous amino acid residue shared by three related RNA binding proteins causative of …

Charting the solid‐state NMR signals of polysaccharides: A database‐driven roadmap

W Zhao, D Debnath, I Gautam… - Magnetic …, 2024 - Wiley Online Library
Solid‐state nuclear magnetic resonance (ssNMR) measurements of intact cell walls and
cellular samples often generate spectra that are difficult to interpret due to the presence of …

Practical use of chemical shift databases for protein solid-state NMR: 2D chemical shift maps and amino-acid assignment with secondary-structure information

KJ Fritzsching, Y Yang, K Schmidt-Rohr… - Journal of biomolecular …, 2013 - Springer
We introduce a Python-based program that utilizes the large database of 13 C and 15 N
chemical shifts in the Biological Magnetic Resonance Bank to rapidly predict the amino acid …

Membrane proteins in their native habitat as seen by solid‐state NMR spectroscopy

LS Brown, V Ladizhansky - Protein Science, 2015 - Wiley Online Library
Membrane proteins play many critical roles in cells, mediating flow of material and
information across cell membranes. They have evolved to perform these functions in the …