A pragmatic guide to enrichment strategies for mass spectrometry–based glycoproteomics

NM Riley, CR Bertozzi, SJ Pitteri - Molecular & Cellular Proteomics, 2021 - ASBMB
Glycosylation is a prevalent, yet heterogeneous modification with a broad range of
implications in molecular biology. This heterogeneity precludes enrichment strategies that …

MS‐based glycomics and glycoproteomics methods enabling isomeric characterization

W Peng, CD Gutierrez Reyes, S Gautam… - Mass spectrometry …, 2023 - Wiley Online Library
Glycosylation is one of the most significant and abundant posttranslational modifications in
mammalian cells. It mediates a wide range of biofunctions, including cell adhesion, cell …

Protein glycosylation in extracellular vesicles: Structural characterization and biological functions

J Macedo-da-Silva, VF Santiago… - Molecular …, 2021 - Elsevier
Extracellular vesicles (EVs) are lipid bilayer-enclosed particles involved in intercellular
communication, delivery of biomolecules from donor to recipient cells, cellular disposal and …

Development of a 96-well plate sample preparation method for integrated N-and O-glycomics using porous graphitized carbon liquid chromatography-mass …

T Zhang, K Madunić, S Holst, J Zhang, C Jin… - Molecular omics, 2020 - pubs.rsc.org
Changes in glycosylation signatures of cells have been associated with pathological
processes in cancer as well as infectious and autoimmune diseases. The current protocols …

Correcting for sparsity and interdependence in glycomics by accounting for glycan biosynthesis

B Bao, BP Kellman, AWT Chiang, Y Zhang… - Nature …, 2021 - nature.com
Glycans are fundamental cellular building blocks, involved in many organismal functions.
Advances in glycomics are elucidating the essential roles of glycans. Still, it remains …

Advances in mass spectrometry‐based glycomics—An update covering the period 2017–2021

KB Donohoo, J Wang, M Goli, A Yu, W Peng… - …, 2022 - Wiley Online Library
The wide variety of chemical properties and biological functions found in proteins is attained
via post‐translational modifications like glycosylation. Covalently bonded to proteins …

Methods for quantification of glycopeptides by liquid separation and mass spectrometry

H Yin, J Zhu - Mass spectrometry reviews, 2023 - Wiley Online Library
Recent advances in analytical techniques provide the opportunity to quantify even low‐
abundance glycopeptides derived from complex biological mixtures, allowing for the …

Big-data glycomics: tools to connect glycan biosynthesis to extracellular communication

BP Kellman, NE Lewis - Trends in biochemical sciences, 2021 - cell.com
Characteristically, cells must sense and respond to environmental cues. Despite the
importance of cell–cell communication, our understanding remains limited and often lacks …

Emerging mass spectrometry-based proteomics methodologies for novel biomedical applications

LK Pino, J Rose, A O'Broin, S Shah… - Biochemical Society …, 2020 - portlandpress.com
Research into the basic biology of human health and disease, as well as translational
human research and clinical applications, all benefit from the growing accessibility and …

Resolving isomeric structures of native glycans by nanoflow porous graphitized carbon chromatography–mass spectrometry

YM She, RY Tam, X Li, M Rosu-Myles… - Analytical …, 2020 - ACS Publications
Characterization of the structural diversity of glycans by liquid chromatography–tandem
mass spectrometry (LC–MS/MS) remains an analytical challenge in large-scale glycomics …