[HTML][HTML] Stabilization of enzymes via immobilization: Multipoint covalent attachment and other stabilization strategies

RC Rodrigues, Á Berenguer-Murcia… - Biotechnology …, 2021 - Elsevier
The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

Enzyme co-immobilization: Always the biocatalyst designers' choice… or not?

S Arana-Peña, D Carballares… - Biotechnology …, 2021 - Elsevier
The increasing relevance of cascade reactions in biocatalysis has sparked a great interest
for enzyme co-immobilization. Enzyme co-immobilization allows access to some kinetic …

Novozym 435: the “perfect” lipase immobilized biocatalyst?

C Ortiz, ML Ferreira, O Barbosa… - Catalysis Science & …, 2019 - pubs.rsc.org
Novozym 435 (N435) is a commercially available immobilized lipase produced by
Novozymes. It is based on immobilization via interfacial activation of lipase B from Candida …

Fine-tuning the micro-environment to optimize the catalytic activity of enzymes immobilized in multivariate metal–organic frameworks

YM Li, J Yuan, H Ren, CY Ji, Y Tao, Y Wu… - Journal of the …, 2021 - ACS Publications
The artificial engineering of an enzyme's structural conformation to enhance its activity is
highly desired and challenging. Anisotropic reticular chemistry, best illustrated in the case of …

Exploitation of food industry waste for high-value products

R Ravindran, AK Jaiswal - Trends in biotechnology, 2016 - cell.com
A growing global population leads to an increasing demand for food production and the
processing industry associated with it and consequently the generation of large amounts of …

Microplastics reduce lipid digestion in simulated human gastrointestinal system

H Tan, T Yue, Y Xu, J Zhao, B Xing - Environmental science & …, 2020 - ACS Publications
Microplastics (MPs) are unavoidably ingested by humans, and their gastrointestinal
processes and impact on lipid digestion are unknown. In the present work, all five MP types …

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres… - Biotechnology …, 2015 - Elsevier
In this review, we detail the efforts performed to couple the purification and the
immobilization of industrial enzymes in a single step. The use of antibodies, the …

The lid domain in lipases: Structural and functional determinant of enzymatic properties

FI Khan, D Lan, R Durrani, W Huan, Z Zhao… - … in bioengineering and …, 2017 - frontiersin.org
Lipases are important industrial enzymes. Most of the lipases operate at lipid–water
interfaces enabled by a mobile lid domain located over the active site. Lid protects the active …

[HTML][HTML] Glutaraldehyde modification of lipases immobilized on octyl agarose beads: Roles of the support enzyme loading and chemical amination of the enzyme on …

P Abellanas-Perez, D Carballares… - International Journal of …, 2023 - Elsevier
Lipase B from Candida antarctica (CALB) and lipase from Thermomyces lanuginosus (TLL)
have been immobilized on octyl agarose at low loading and at a loading exceeding the …

[HTML][HTML] Biocatalytic production of biolubricants: Strategies, problems and future trends

RRC Monteiro, Á Berenguer-Murcia… - Biotechnology …, 2023 - Elsevier
The increasing worries by the inadequate use of energy and the preservation of nature are
promoting an increasing interest in the production of biolubricants. After discussing the …