Base editing—the introduction of single-nucleotide variants (SNVs) into DNA or RNA in
living cells—is one of the most recent advances in the field of genome editing. As around …

Next-generation sequencing technology has rapidly accelerated the discovery of genetic
variants of interest in individuals with rare diseases. However, showing that these variants …

Dietary mono-unsaturated fatty acids (MUFAs) are linked to longevity in several species. But
the mechanisms by which MUFAs extend lifespan remain unclear. Here we show that an …

Aging is characterized by a global decline in physiological function. However, by
constructing a complete single-cell gene expression atlas, we find that Caenorhabditis …

Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome
engineering in single cells and whole organisms. In the research arena, versatile CRISPR …

A robust genome editing pipeline is critical to the vitality of a modern genetic laboratory.
Previous studies have shown that Cas9 ribonucleoprotein (RNP)-based editing can be …

Genome editing offers promising solutions to genetic disorders by editing DNA sequences
or modulating gene expression. The clustered regularly interspaced short palindromic …

Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …

Melting and fast cooling double stranded DNA donor molecules prior to injection
dramatically increases the frequency of homology-directed repair for edits such as insertions …

The multispanning membrane protein ATG9A is a scramblase that flips phospholipids
between the two membrane leaflets, thus contributing to the expansion of the phagophore …