The ABCF proteins in Escherichia coli individually cope with 'hard-to-translate' nascent peptide sequences
Y Chadani, S Yamanouchi, E Uemura… - Nucleic Acids …, 2024 - academic.oup.com
Organisms possess a wide variety of proteins with diverse amino acid sequences, and their
synthesis relies on the ribosome. Empirical observations have led to the misconception that …
synthesis relies on the ribosome. Empirical observations have led to the misconception that …
An antibiotic preorganized for ribosomal binding overcomes antimicrobial resistance
We report the design conception, chemical synthesis, and microbiological evaluation of the
bridged macrobicyclic antibiotic cresomycin (CRM), which overcomes evolutionarily diverse …
bridged macrobicyclic antibiotic cresomycin (CRM), which overcomes evolutionarily diverse …
Structural basis of Cfr-mediated antimicrobial resistance and mechanisms to evade it
EV Aleksandrova, KJY Wu, BIC Tresco… - Nature Chemical …, 2024 - nature.com
The bacterial ribosome is an essential drug target as many clinically important antibiotics
bind and inhibit its functional centers. The catalytic peptidyl transferase center (PTC) is …
bind and inhibit its functional centers. The catalytic peptidyl transferase center (PTC) is …
Resolution of ribosomal stalling by EF-P and ABCF ATPases YfmR and YkpA/YbiT
H Takada, K Fujiwara, GC Atkinson… - Nucleic Acids …, 2024 - academic.oup.com
Efficiency of protein synthesis on the ribosome is strongly affected by the amino acid
composition of the assembled amino acid chain. Challenging sequences include proline …
composition of the assembled amino acid chain. Challenging sequences include proline …
Cryo-EM studies of the four E. coli paralogs establish ABCF proteins as master plumbers of the peptidyl-transferase center of the ribosome
The genomes of most mesophilic organisms encode multiple A TP-B inding C assette F
(ABCF) proteins. EttA, one of four E. coli paralogs, regulates synthesis of the first peptide …
(ABCF) proteins. EttA, one of four E. coli paralogs, regulates synthesis of the first peptide …
Machine learning-based classification reveals distinct clusters of non-coding genomic allelic variations associated with Erm-mediated antibiotic resistance
The erythromycin resistance RNA methyltransferase (erm) confers cross-resistance to all
therapeutically important macrolides, lincosamides, and streptogramins (MLS phenotype) …
therapeutically important macrolides, lincosamides, and streptogramins (MLS phenotype) …
The ABCF proteins in Escherichia coli individually alleviate nascent peptide-induced noncanonical translations.
Y Chadani, E Uemura, K Yamazaki, M Kurihara… - bioRxiv, 2023 - biorxiv.org
Organisms possess a wide variety of proteins with a diverse repertoire of amino acid
sequences, and their synthesis relies on the ribosome. Empirical observations have led to …
sequences, and their synthesis relies on the ribosome. Empirical observations have led to …
Comparative genetic, biochemical, and biophysical analyses of the four E. coli ABCF paralogs support distinct functions related to mRNA translation
Multiple paralogous ABCF ATPases are encoded in most genomes, but the physiological
functions remain unknown for most of them. We herein compare the four Escherichia coli …
functions remain unknown for most of them. We herein compare the four Escherichia coli …
Global regulation via modulation of ribosome pausing by EttA
F Ousalem, S Ngo, T Oiffer, A Omairi-Nasser, M Hamon… - bioRxiv, 2023 - biorxiv.org
Having multiple rounds of translation of the same mRNA is a key feature of the Central
Dogma that creates substantial dynamic complexities along with opportunities for regulation …
Dogma that creates substantial dynamic complexities along with opportunities for regulation …
[HTML][HTML] Structural basis of Cfr-mediated antimicrobial resistance and mechanisms for its evasion
The ribosome is an essential drug target as many classes of clinically important antibiotics
bind and inhibit its functional centers. The catalytic peptidyl transferase center (PTC) is …
bind and inhibit its functional centers. The catalytic peptidyl transferase center (PTC) is …