In this paper, we provide a general protocol for labeling proteins with the membrane-
permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder–ethanedithiol …

Calmodulin (CaM) mediates a wide range of biological responses to changes in intracellular
Ca2+ concentrations through its calcium-dependent binding affinities to numerous target …

Shigella flexneri uses its type III secretion apparatus (TTSA) to inject host-altering proteins
into targeted eukaryotic cells. The TTSA is composed of a basal body and an exposed …

We report a single-molecule fluorescence resonance energy transfer (FRET) and
polarization study of conformational dynamics of calmodulin (CaM) interacting with a target …

Fluorophore-assisted light inactivation (FALI) permits the targeted inactivation of tagged
proteins and, when used with cell-permeable multiuse affinity probes (MAPs), offers …

A short synthesis of EDTA-based metal chelates that can be attached to the cysteine residue
of a protein via a disulfide bond is described. The complexes were used after coordination of …

Under conditions of oxidative stress, the 20S proteasome plays a critical role in maintaining
cellular homeostasis through the selective degradation of oxidized and damaged proteins …

Metal ions play an essential role in several cellular functions. Calcium is a ubiquitous
regulator and is involved in numerous physiological processes. A class of proteins have …

The rotavirus NSP5 protein directs the formation of viroplasm-like structures (VLS) and is
required for viroplasm formation within infected cells. In this report, we have defined signals …

New methods are needed to selectively label proteins in a manner that minimally perturbs
their structures and functions. We have developed a 'small molecule'-based labelling …