Light sheet fluorescence microscopy

EHK Stelzer, F Strobl, BJ Chang, F Preusser… - Nature Reviews …, 2021 - nature.com
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

The ImageJ ecosystem: An open platform for biomedical image analysis

J Schindelin, CT Rueden, MC Hiner… - Molecular …, 2015 - Wiley Online Library
Technology in microscopy advances rapidly, enabling increasingly affordable, faster, and
more precise quantitative biomedical imaging, which necessitates correspondingly more …

SpaceM reveals metabolic states of single cells

L Rappez, M Stadler, S Triana, RM Gathungu… - Nature …, 2021 - nature.com
A growing appreciation of the importance of cellular metabolism and revelations concerning
the extent of cell–cell heterogeneity demand metabolic characterization of individual cells …

Pathogenic variants damage cell composition and single cell transcription in cardiomyopathies

D Reichart, EL Lindberg, H Maatz, AMA Miranda… - Science, 2022 - science.org
Pathogenic variants in genes that cause dilated cardiomyopathy (DCM) and arrhythmogenic
cardiomyopathy (ACM) convey high risks for the development of heart failure through …

Coordinated cellular neighborhoods orchestrate antitumoral immunity at the colorectal cancer invasive front

CM Schürch, SS Bhate, GL Barlow, DJ Phillips, L Noti… - Cell, 2020 - cell.com
Antitumoral immunity requires organized, spatially nuanced interactions between
components of the immune tumor microenvironment (iTME). Understanding this coordinated …

Virtual histological staining of unlabelled tissue-autofluorescence images via deep learning

Y Rivenson, H Wang, Z Wei, K de Haan… - Nature biomedical …, 2019 - nature.com
The histological analysis of tissue samples, widely used for disease diagnosis, involves
lengthy and laborious tissue preparation. Here, we show that a convolutional neural network …

Dendritic action potentials and computation in human layer 2/3 cortical neurons

A Gidon, TA Zolnik, P Fidzinski, F Bolduan, A Papoutsi… - Science, 2020 - science.org
The active electrical properties of dendrites shape neuronal input and output and are
fundamental to brain function. However, our knowledge of active dendrites has been almost …

Deep profiling of mouse splenic architecture with CODEX multiplexed imaging

Y Goltsev, N Samusik, J Kennedy-Darling, S Bhate… - Cell, 2018 - cell.com
A highly multiplexed cytometric imaging approach, termed co-detection by indexing
(CODEX), is used here to create multiplexed datasets of normal and lupus (MRL/lpr) murine …

Deep learning enables cross-modality super-resolution in fluorescence microscopy

H Wang, Y Rivenson, Y Jin, Z Wei, R Gao… - Nature …, 2019 - nature.com
We present deep-learning-enabled super-resolution across different fluorescence
microscopy modalities. This data-driven approach does not require numerical modeling of …

BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis

K McArthur, LW Whitehead, JM Heddleston, L Li… - Science, 2018 - science.org
INTRODUCTION There has been an explosion of interest in the role of cell death pathways
and damage-associated molecular pattern (DAMP) signaling in shaping inflammatory and …