Point-of-care-testing of nucleic acids by microfluidics

L Zhang, B Ding, Q Chen, Q Feng, L Lin… - TrAC Trends in Analytical …, 2017 - Elsevier
Nucleic acid detection offers critical information in diagnosing pathogen infection and
monitoring disease treatment. Point-of-care testing (POCT), with ideal characteristics such …

Contributions and challenges of high throughput qPCR for determining antimicrobial resistance in the environment: a critical review

H Waseem, S Jameel, J Ali, H Saleem Ur Rehman… - Molecules, 2019 - mdpi.com
Expansion in whole genome sequencing and subsequent increase in antibiotic resistance
targets have paved the way of high throughput qPCR (HT-qPCR) for analyzing hundreds of …

Reagents-loaded, automated assay that integrates recombinase-aided amplification and Cas12a nucleic acid detection for a point-of-care test

Y Chen, Y Mei, X Zhao, X Jiang - Analytical chemistry, 2020 - ACS Publications
Point-of-care testing for nucleic acid that combines amplification and readout is in great
demand. This report integrates recombinase-aided amplification (RAA) with the clustered …

Waterborne pathogens detection technologies: advances, challenges, and future perspectives

YL Oon, YS Oon, M Ayaz, M Deng, L Li… - Frontiers in …, 2023 - frontiersin.org
The World Health Organization (WHO) estimated that pathogens like Escherichia coli,
primarily linked to food and water contamination, are associated with 485,000 deaths from …

[HTML][HTML] A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Enterococcus spp. in water

R Martzy, C Kolm, K Brunner, RL Mach, R Krska… - Water research, 2017 - Elsevier
Faecal pollution of water and the resulting potential presence of human enteric pathogens is
a predominant threat to public health. Microbiological water quality can be assessed by the …

Rapid and in-situ detection of fecal indicator bacteria in water using simple DNA extraction and portable loop-mediated isothermal amplification (LAMP) PCR methods

S Lee, VSL Khoo, CAD Medriano, T Lee, SY Park… - Water research, 2019 - Elsevier
Contamination of water by fecal matter and potential human enteric pathogens is a serious
health concern. Microbiological water quality has been assessed by conventional culture …

Lab on a smartphone (LOS): A smartphone-integrated, plasmonic-enhanced optoelectrowetting (OEW) platform for on-chip water quality monitoring through LAMP …

SK Thio, SW Bae, SY Park - Sensors and Actuators B: Chemical, 2022 - Elsevier
We present a low-cost lab-on-a-smartphone (LOS) platform for rapid and in-situ monitoring
of fecal indicator bacteria (FIB) such as Escherichia coli (E. coli) to examine the presence …

Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis

R Martzy, C Kolm, R Krska, RL Mach… - Analytical and …, 2019 - Springer
Molecular diagnostic tools in the field of food and water quality analysis are becoming
increasingly widespread. Usually, based on DNA amplification techniques such as …

Microfluidic-based nucleic acid amplification systems in microbiology

L Gorgannezhad, H Stratton, NT Nguyen - Micromachines, 2019 - mdpi.com
Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this
research area has had a broad range of applications. Novel and innovative strategies for …

Molecular diagnostic tools applied for assessing microbial water quality

L Paruch - International Journal of Environmental Research and …, 2022 - mdpi.com
Microbial water quality is of vital importance for human, animal, and environmental health.
Notably, pathogenically contaminated water can result in serious health problems, such as …