Environmental DNA metabarcoding: Transforming how we survey animal and plant communities
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …
[HTML][HTML] The future of biotic indices in the ecogenomic era: Integrating (e) DNA metabarcoding in biological assessment of aquatic ecosystems
The bioassessment of aquatic ecosystems is currently based on various biotic indices that
use the occurrence and/or abundance of selected taxonomic groups to define ecological …
use the occurrence and/or abundance of selected taxonomic groups to define ecological …
Counting with DNA in metabarcoding studies: How should we convert sequence reads to dietary data?
Advances in DNA sequencing technology have revolutionized the field of molecular analysis
of trophic interactions, and it is now possible to recover counts of food DNA sequences from …
of trophic interactions, and it is now possible to recover counts of food DNA sequences from …
Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment
F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …
numerous studies have investigated the consistency of this technique with traditional …
Scrutinizing key steps for reliable metabarcoding of environmental samples
Metabarcoding of environmental samples has many challenges and limitations that require
carefully considered laboratory and analysis workflows to ensure reliable results. We …
carefully considered laboratory and analysis workflows to ensure reliable results. We …
How quantitative is metabarcoding: A meta‐analytical approach
Metabarcoding has been used in a range of ecological applications such as taxonomic
assignment, dietary analysis and the analysis of environmental DNA. However, after a …
assignment, dietary analysis and the analysis of environmental DNA. However, after a …
Non‐specific amplification compromises environmental DNA metabarcoding with COI
Metabarcoding extra‐organismal DNA from environmental samples is now a key technique
in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when …
in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when …
Uses and misuses of environmental DNA in biodiversity science and conservation
ME Cristescu, PDN Hebert - Annual Review of Ecology …, 2018 - annualreviews.org
The study of environmental DNA (eDNA) has the potential to revolutionize biodiversity
science and conservation action by enabling the census of species on a global scale in near …
science and conservation action by enabling the census of species on a global scale in near …
An assessment of minimum sequence copy thresholds for identifying and reducing the prevalence of artefacts in dietary metabarcoding data
LE Drake, JP Cuff, RE Young… - Methods in Ecology …, 2022 - Wiley Online Library
Metabarcoding provides a powerful tool for investigating biodiversity and trophic
interactions, but the high sensitivity of this methodology makes it vulnerable to errors …
interactions, but the high sensitivity of this methodology makes it vulnerable to errors …
Can DNA-based ecosystem assessments quantify species abundance? Testing primer bias and biomass—sequence relationships with an innovative metabarcoding …
V Elbrecht, F Leese - PloS one, 2015 - journals.plos.org
Metabarcoding is an emerging genetic tool to rapidly assess biodiversity in ecosystems. It
involves high-throughput sequencing of a standard gene from an environmental sample and …
involves high-throughput sequencing of a standard gene from an environmental sample and …