Zero-Mode waveguide nanowells for single-molecule detection in living cells

S Yang, N Klughammer, A Barth, ME Tanenbaum… - ACS …, 2023 - ACS Publications
Single-molecule fluorescence imaging experiments generally require sub-nanomolar
protein concentrations to isolate single protein molecules, which makes such experiments …

Revealing the Binding Events of Single Proteins on Exosomes Using Nanocavity Antennas beyond Zero-Mode Waveguides

Q Gao, P Zang, J Li, W Zhang, Z Zhang… - … Applied Materials & …, 2023 - ACS Publications
Exosomes (EXOs) play a crucial role in biological action mechanisms. Understanding the
biological process of single-molecule interactions on the surface of the EXO membrane is …

Single Effective Complex Loading into Zero-Mode Waveguides Optimized with Fluorescence Evaluation at Quenching and Accumulation Checkpoints

L Wang, P Zang, J Li, Z Zhang, C Li… - … Applied Materials & …, 2024 - ACS Publications
Single-molecule detection with high accuracy and specialty plays an important role in
biomedical diagnosis and screening. Zero-mode waveguides (ZMWs) enable the possibility …

Zero-mode waveguides and nanopore-based sequencing technologies accelerate single-molecule studies

R Iizuka, H Yamazaki, S Uemura - Biophysics and Physicobiology, 2022 - jstage.jst.go.jp
Single-molecule technologies can provide detailed information regarding molecular
mechanisms and interactions that cannot easily be studied on the bulk scale; generally …

Analysis tools for single-monomer measurements of self-assembly processes

M Hoyer, AH Crevenna, R Kitel, K Willems, M Czub… - Scientific Reports, 2022 - nature.com
Protein assembly plays an important role throughout all phyla of life, both physiologically
and pathologically. In particular, aggregation and polymerization of proteins are key …

Combined estimation of presepsin and gelsolin might improve the diagnostic validity of clinical scoring to predict and stratify sepsis in non-sepsis surgical ICU patient

HA Shehab, AM Eid, YS Dabour - Egyptian Journal of Anaesthesia, 2024 - Taylor & Francis
Objectives This study evaluates the ability of serum presepsin (PSEP) and gelsolin (GSN)
levels estimated in blood samples obtained at the admission of sepsis-free patients to …